Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prenylcysteine oxidase 1 (PCYOX1) Standard Curve.)
Mouse Prenylcysteine Oxidase 1 (PCYOX1) ELISA Kit | PCYOX1 elisa kit
Mouse Prenylcysteine Oxidase 1 (PCYOX1) ELISA Kit
Gene Names
Pcyox1; PCL1; Pcly; AI115532; AI448340; 1200015P13Rik
Reactivity
Mouse
Synonyms
Prenylcysteine Oxidase 1 (PCYOX1); Mouse Prenylcysteine Oxidase 1 (PCYOX1) ELISA Kit; PCYOX1; KIAA0908; PCL1; prenylcysteine lyase; PCYOX1 elisa kit
Reactivity
Mouse
Specificity
Mouse Prenylcysteine oxidase 1 (PCYOX1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PCYOX1. No significant cross-reactivity or interference between Mouse PCYOX1 and analogues was observed.
Sequence Length
505
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prenylcysteine oxidase 1 (PCYOX1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prenylcysteine oxidase 1 (PCYOX1) Standard Curve.)
Related Product Information for PCYOX1 elisa kit
Description: This Mouse Prenylcysteine oxidase 1 (PCYOX1) ELISA Kit employs a two-site sandwich ELISA to quantitate PCYOX1 in samples. An antibody specific for PCYOX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCYOX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCYOX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCYOX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Prenylcysteine is released during the degradation of prenylated proteins. PCYOX1 catalyzes the degradation of prenylcysteine to yield free cysteines and a hydrophobic isoprenoid product. The deduced 505-amino acid protein contains a predicted signal sequence, several transmembrane regions, and potential N-glycosylation sites. Northern blot analysis detected a 6-kb transcript in heart, kidney, liver, colon, placenta, skeletal muscle, and pancreas, with low expression in brain and no expression in spleen. The 3-prime untranslated region contains several SINEs, including Alu and MIR sequences. The predicted protein has 506 amino acids. RT-PCR ELISA detected high to moderate expression in all adult and fetal human tissues and all specific brain regions tested.
Background: Prenylcysteine is released during the degradation of prenylated proteins. PCYOX1 catalyzes the degradation of prenylcysteine to yield free cysteines and a hydrophobic isoprenoid product. The deduced 505-amino acid protein contains a predicted signal sequence, several transmembrane regions, and potential N-glycosylation sites. Northern blot analysis detected a 6-kb transcript in heart, kidney, liver, colon, placenta, skeletal muscle, and pancreas, with low expression in brain and no expression in spleen. The 3-prime untranslated region contains several SINEs, including Alu and MIR sequences. The predicted protein has 506 amino acids. RT-PCR ELISA detected high to moderate expression in all adult and fetal human tissues and all specific brain regions tested.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prenylcysteine oxidase isoform 1
NCBI Official Synonym Full Names
prenylcysteine oxidase 1
NCBI Official Symbol
Pcyox1
NCBI Official Synonym Symbols
PCL1; Pcly; AI115532; AI448340; 1200015P13Rik
NCBI Protein Information
prenylcysteine oxidase
UniProt Protein Name
Prenylcysteine oxidase
Protein Family
UniProt Gene Name
Pcyox1
UniProt Synonym Gene Names
Kiaa0908
Uniprot Description
Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine ().
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Product Notes
The Mouse PCYOX1 pcyox1 (Catalog #AAA9717392) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717392 ELISA Kit recognizes Mouse PCYOX1. It is sometimes possible for the material contained within the vial of "Prenylcysteine Oxidase 1 (PCYOX1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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