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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Mouse PRELI domain-containing protein 2 (PRELID2) Standard Curve.)

Mouse PRELI Domain-Containing Protein 2 (PRELID2) ELISA Kit | PRELID2 elisa kit

Mouse PRELI Domain-Containing Protein 2 (PRELID2) ELISA Kit

Gene Names
Prelid2; 1700003A01Rik; C330008K14Rik
Reactivity
Mouse
Synonyms
PRELI Domain-Containing Protein 2 (PRELID2); Mouse PRELI Domain-Containing Protein 2 (PRELID2) ELISA Kit; PRELID2; FLJ38376; MGC21644; PRELID2 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
Mouse PRELI domain-containing protein 2 (PRELID2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRELID2. No significant cross-reactivity or interference between Mouse PRELID2 and analogues was observed.
Sequence Length
177
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse PRELI domain-containing protein 2 (PRELID2) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Mouse PRELI domain-containing protein 2 (PRELID2) Standard Curve.)
Related Product Information for PRELID2 elisa kit
Description: This Mouse PRELI domain-containing protein 2 (PRELID2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRELID2 in samples. An antibody specific for PRELID2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRELID2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRELID2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRELID2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Prelid2, which belongs to the PRELI domain containing family, is identified as a conserved evolution gene. The expression and regulation during embryonic development of the prelid2 gene is unknown. prelid2 gene expression were found in midbrain, spinal cord, optic eminence, otic vesicle and tail at E9.5 and E10.5 embryos, in forebrain, hindbrain, heart, lung, liver and kidney at E13.5 and E15.5 embryos. Real-time quantitative RT-PCR results verified the expression pattern in the four major mouse organs, brain, heart, lung, and liver during organs differentiation and formation. Bisulfite sequencing illustrated the consistent result of expression and its unmethylation status in the genomic promoter region at E12.5, E18.5, and new born. Thus, the prelid2 gene is a widely-spread, persistently expressed and unmethylated gene in mouse embryonic development.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
PRELI domain-containing protein 2
NCBI Official Synonym Full Names
PRELI domain containing 2
NCBI Official Symbol
Prelid2
NCBI Official Synonym Symbols
1700003A01Rik; C330008K14Rik
NCBI Protein Information
PRELI domain-containing protein 2
UniProt Protein Name
PRELI domain-containing protein 2
UniProt Gene Name
Prelid2
UniProt Entry Name
PRLD2_MOUSE

Research Articles on PRELID2

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Product Notes

The Mouse PRELID2 prelid2 (Catalog #AAA9717297) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717297 ELISA Kit recognizes Mouse PRELID2. It is sometimes possible for the material contained within the vial of "PRELI Domain-Containing Protein 2 (PRELID2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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