Mouse Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) ELISA Kit | PAFAH2 elisa kit

Mouse Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) ELISA Kit

Cat. Num. AAA2022001

• Gene Names: PAFAH2; HSD-PLA2
• Reactivity: Mouse
• Synonyms: Platelet Activating Factor Acetylhydrolase 2 (PAFAH2); Mouse Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) ELISA Kit; SD-PLA2; Serine-dependent phospholipase A2; Platelet-activating factor acetylhydrolase 2; cytoplasmic; PAFAH2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Platelet Activating Factor Acetylhydrolase 2 (PAFAH2).; No significant cross-reactivity or interference between Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) and analogues was observed.
• Sequence Length: 392

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 31.25-2000pg/mL
• Sensitivity: < 14.1pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAFAH2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Activating Factor Acetylhydrolase 2 (PAFAH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Activating Factor Acetylhydrolase 2 (PAFAH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Activating Factor Acetylhydrolase 2 (PAFAH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PAFAH2 elisa kit

Enzyme & Kinase; Metabolic pathway; Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 1765864
• NCBI GeneID: 5051
• Molecular Weight: 44,036 Da
• NCBI Official Full Name: platelet-activating factor acetylhydrolase 2
• NCBI Official Synonym Full Names: platelet-activating factor acetylhydrolase 2, 40kDa
• NCBI Official Symbol: PAFAH2
• NCBI Official Synonym Symbols: HSD-PLA2
• NCBI Protein Information: platelet-activating factor acetylhydrolase 2, cytoplasmic
• UniProt Protein Name: Platelet-activating factor acetylhydrolase 2, cytoplasmic
• Protein Family: Platelet-activating factor acetylhydrolase
• UniProt Gene Name: PAFAH2
• UniProt Synonym Gene Names: SD-PLA2; hSD-PLA2
• UniProt Entry Name: PAFA2_HUMAN

NCBI Description

This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme. [provided by RefSeq, Jul 2008]

Uniprot Description

PAFAH2: Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzyme. Belongs to the serine esterase family.

Protein type: EC 3.1.1.47; Lipid-binding; Lipid Metabolism - ether lipid; Hydrolase

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: cytoplasm

Molecular Function: phospholipid binding; 1-alkyl-2-acetylglycerophosphocholine esterase activity

Biological Process: lipid metabolic process; lipid catabolic process; negative regulation of apoptosis