Mouse Phospholipase A2, Lipoprotein Associated (LpPLA2) ELISA Kit | LpPLA2 elisa kit

Mouse Phospholipase A2, Lipoprotein Associated (LpPLA2) ELISA Kit

Cat. Num. AAA2702624

• Gene Names: PLA2G2A; MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
• Reactivity: Mouse
• Synonyms: Phospholipase A2; Lipoprotein Associated (LpPLA2); Mouse Phospholipase A2; Lipoprotein Associated (LpPLA2) ELISA Kit; PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase; Plasma; Group VII; LDL-associated phospholipase A2; LpPLA2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Lipoprotein Associated (LpPLA2).; No significant cross-reactivity or interference between Phospholipase A2, Lipoprotein Associated (LpPLA2) and analogues was observed.
• Sequence Length: 144

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.137ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2, Lipoprotein Associated (LpPLA2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2, Lipoprotein Associated (LpPLA2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LpPLA2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Lipoprotein Associated (LpPLA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Lipoprotein Associated (LpPLA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Lipoprotein Associated (LpPLA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Lipoprotein Associated (LpPLA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 239915991
• NCBI GeneID: 5320
• NCBI Accession #: NP_001155201.1
• NCBI GenBank Nucleotide #: NM_001161729.1
• UniProt Accession #: P14555; Q6DN24; Q6IBD9; Q9UCD2; A8K5I7
• Molecular Weight: 16,083 Da
• NCBI Official Full Name: phospholipase A2, membrane associated
• NCBI Official Synonym Full Names: phospholipase A2 group IIA
• NCBI Official Symbol: PLA2G2A
• NCBI Official Synonym Symbols: MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
• NCBI Protein Information: phospholipase A2, membrane associated
• UniProt Protein Name: Phospholipase A2, membrane associated
• UniProt Gene Name: PLA2G2A
• UniProt Synonym Gene Names: PLA2B; PLA2L; RASF-A; NPS-PLA2

NCBI Description

The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:2925633). Thought to participate in the regulation of phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Independent of its catalytic activity, acts as a ligand for integrins (PubMed:18635536, PubMed:25398877). Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 (PubMed:18635536, PubMed:25398877). Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:25398877). Induces cell proliferation in an integrin-dependent manner (PubMed:18635536).