Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphatidylinositol-glycan biosynthesis class F protein (PIGF) Standard Curve.)
Mouse Phosphatidylinositol-Glycan Biosynthesis Class F Protein (PIGF) ELISA Kit | PIGF elisa kit
Mouse Phosphatidylinositol-Glycan Biosynthesis Class F Protein (PIGF) ELISA Kit
Reactivity
Mouse
Synonyms
Phosphatidylinositol-Glycan Biosynthesis Class F Protein (PIGF); Mouse Phosphatidylinositol-Glycan Biosynthesis Class F Protein (PIGF) ELISA Kit; PIGF; MGC32646; MGC33136; PIGF elisa kit
Reactivity
Mouse
Specificity
Mouse Phosphatidylinositol-glycan biosynthesis class F protein (PIGF) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PIGF. No significant cross-reactivity or interference between Mouse PIGF and analogues was observed.
Sequence Length
219
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphatidylinositol-glycan biosynthesis class F protein (PIGF) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phosphatidylinositol-glycan biosynthesis class F protein (PIGF) Standard Curve.)
Related Product Information for PIGF elisa kit
Description: This Mouse Phosphatidylinositol-glycan biosynthesis class F protein (PIGF) ELISA Kit employs a two-site sandwich ELISA to quantitate PIGF in samples. An antibody specific for PIGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIGF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PIGF encodes 1 of the enzymes involved in glycosylphosphatidylinositol (GPI) anchor biosynthesis. The primary biochemical defect in paroxysmal nocturnal hemoglobinuria (PNH), an acquired hematologic disorder with protean clinical manifestations including recurrent episodes of hemolysis, infections, and venous thrombosis, involves defective synthesis of GPI anchors that attach certain proteins to the cell surface membrane. Proteins with appropriate C-terminal signal peptides are transferred to the preformed glycolipid anchor in the endoplasmic reticulum and transported to the cell surface as a single unit; in PNH, there is a defect in GPI-anchor biosynthesis and an absence of all surface proteins that use this anchoring motif.
Background: PIGF encodes 1 of the enzymes involved in glycosylphosphatidylinositol (GPI) anchor biosynthesis. The primary biochemical defect in paroxysmal nocturnal hemoglobinuria (PNH), an acquired hematologic disorder with protean clinical manifestations including recurrent episodes of hemolysis, infections, and venous thrombosis, involves defective synthesis of GPI anchors that attach certain proteins to the cell surface membrane. Proteins with appropriate C-terminal signal peptides are transferred to the preformed glycolipid anchor in the endoplasmic reticulum and transported to the cell surface as a single unit; in PNH, there is a defect in GPI-anchor biosynthesis and an absence of all surface proteins that use this anchoring motif.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phosphatidylinositol-glycan biosynthesis class F protein
NCBI Official Synonym Full Names
phosphatidylinositol glycan anchor biosynthesis, class F
NCBI Official Symbol
Pigf
NCBI Protein Information
phosphatidylinositol-glycan biosynthesis class F protein
UniProt Protein Name
Phosphatidylinositol-glycan biosynthesis class F protein
Protein Family
UniProt Gene Name
Pigf
UniProt Synonym Gene Names
PIG-F
UniProt Entry Name
PIGF_MOUSE
Uniprot Description
PIGF: Involved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI. Belongs to the PIGF family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane
Molecular Function: phosphotransferase activity, for other substituted phosphate groups
Biological Process: GPI anchor biosynthetic process
Similar Products
Product Notes
The Mouse PIGF pigf (Catalog #AAA9717364) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717364 ELISA Kit recognizes Mouse PIGF. It is sometimes possible for the material contained within the vial of "Phosphatidylinositol-Glycan Biosynthesis Class F Protein (PIGF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
