Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peptide chain release factor 1, mitochondrial (MTRF1) Standard Curve.)
Mouse Peptide Chain Release Factor 1, Mitochondrial (MTRF1) ELISA Kit | MTRF1 elisa kit
Mouse Peptide Chain Release Factor 1, Mitochondrial (MTRF1) ELISA Kit
Gene Names
Mtrf1; MtRF-1; A830062K05Rik
Reactivity
Mouse
Synonyms
Peptide Chain Release Factor 1; Mitochondrial (MTRF1); Mouse Peptide Chain Release Factor 1; Mitochondrial (MTRF1) ELISA Kit; MTRF1; RP11-74J13.3; MGC47721; MRF1; MTTRF1; RF1; OTTHUMP00000018316; mitochontrial peptide chain release factor 1; MTRF1 elisa kit
Reactivity
Mouse
Specificity
Mouse Peptide chain release factor 1, mitochondrial (MTRF1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MTRF1. No significant cross-reactivity or interference between Mouse MTRF1 and analogues was observed.
Sequence Length
446
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peptide chain release factor 1, mitochondrial (MTRF1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peptide chain release factor 1, mitochondrial (MTRF1) Standard Curve.)
Related Product Information for MTRF1 elisa kit
Description: This Mouse Peptide chain release factor 1, mitochondrial (MTRF1) ELISA Kit employs a two-site sandwich ELISA to quantitate MTRF1 in samples. An antibody specific for MTRF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MTRF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTRF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTRF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The Mitochondrial Translation Release Factor 1 encoded by MTRF1 was determined by in silico methods to be a mitochondrial protein with similarity to the peptide chain release factors (RFs) discovered in bacteria and yeast. The peptide chain release factors direct the termination of translation in response to the peptide chain termination codons. Initially thought to have a role in the termination of mitochondria protein synthesis, a recent publication found no mitochondrial translation release functionality. Multiple alternatively spliced transcript variants have been suggested by mRNA and EST data; however, their full-length natures are not clear.
Background: The Mitochondrial Translation Release Factor 1 encoded by MTRF1 was determined by in silico methods to be a mitochondrial protein with similarity to the peptide chain release factors (RFs) discovered in bacteria and yeast. The peptide chain release factors direct the termination of translation in response to the peptide chain termination codons. Initially thought to have a role in the termination of mitochondria protein synthesis, a recent publication found no mitochondrial translation release functionality. Multiple alternatively spliced transcript variants have been suggested by mRNA and EST data; however, their full-length natures are not clear.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
peptide chain release factor 1, mitochondrial
NCBI Official Synonym Full Names
mitochondrial translational release factor 1
NCBI Official Symbol
Mtrf1
NCBI Official Synonym Symbols
MtRF-1; A830062K05Rik
NCBI Protein Information
peptide chain release factor 1, mitochondrial
UniProt Protein Name
Peptide chain release factor 1, mitochondrial
UniProt Gene Name
Mtrf1
UniProt Synonym Gene Names
MRF-1; MtRF-1
UniProt Entry Name
RF1M_MOUSE
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Product Notes
The Mouse MTRF1 mtrf1 (Catalog #AAA9717516) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717516 ELISA Kit recognizes Mouse MTRF1. It is sometimes possible for the material contained within the vial of "Peptide Chain Release Factor 1, Mitochondrial (MTRF1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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