Mouse HNRPM / HNRNPM Monoclonal Antibody | anti-HNRPM antibody

Mouse Monoclonal [clone 3F7] (IgG2a,k) to Human HNRPM / HNRNPM

Cat. Num. AAA245794

• Gene Names: HNRNPM; CEAR; HNRPM; HTGR1; NAGR1; HNRPM4; HNRNPM4; hnRNP M
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: HNRPM / HNRNPM; Monoclonal Antibody; Mouse Monoclonal [clone 3F7] (IgG2a; k) to Human HNRPM / HNRNPM; Anti-HNRPM / HNRNPM Antibody (clone 3F7) IHC-plus; HNRNPM; CEA receptor; CEAR; HnRNA-binding protein M4; HNRNPM4; HNRPM4; N-acetylglucosamine receptor 1; NAGR1; HnRNP M; HNRPM; HTGR1; Human HNRPM; anti-HNRPM antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3F7
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 730

• Applicable Applications for anti-HNRPM antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB; Usage: Western Blot (Cell lysate) - positive control PC-12. Western Blot (Cell lysate) - positive control HepG2. Western Blot (Cell lysate) - positive control NIH/3T3. Immunofluorescence (10 ug/ml) - positive control HepG2 cells. Sandwich ELISA (Recombinant...
• Target Species: Human
• Immunogen Description: HNRPM (NP_005959, 17 a.a. ~ 113 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: HNRPM / HNRNPM antibody was raised against hNRPM (NP_005959, 17 a.a. ~ 113 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-HNRNPM / HNRPM antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-HNRNPM / HNRPM antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to HNRPM on HepG2 cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(HNRPM monoclonal antibody clone 3F7. Western blot of HNRPM expression in PC-12.)

Western Blot (WB)

(HNRPM monoclonal antibody clone 3F7. Western blot of HNRPM expression in NIH/3T3.)

Western Blot (WB)

(HNRPM monoclonal antibody clone 3F7 Western blot of HNRPM expression in HepG2.)

ELISA (EIA)

(Detection limit for recombinant GST tagged HNRPM is approximately 0.1 ng/ml as a capture antibody.)

Related Product Information for anti-HNRPM antibody

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm.

NCBI and Uniprot Product Information

• NCBI GI #: 14141152
• NCBI GeneID: 4670
• NCBI Accession #: NP_005959.2
• NCBI GenBank Nucleotide #: NM_005968.4
• UniProt Accession #: P52272; Q15584; Q8WZ44; Q96H56; Q9BWL9; Q9Y492
• Molecular Weight: – Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein M isoform a
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein M
• NCBI Official Symbol: HNRNPM
• NCBI Official Synonym Symbols: CEAR; HNRPM; HTGR1; NAGR1; HNRPM4; HNRNPM4; hnRNP M
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein M; CEA receptor; hnRNA-binding protein M4; N-acetylglucosamine receptor 1; heterogenous nuclear ribonucleoprotein M4
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein M
• UniProt Gene Name: HNRNPM
• UniProt Synonym Gene Names: HNRPM; NAGR1; hnRNP M
• UniProt Entry Name: HNRPM_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. This protein also constitutes a monomer of the N-acetylglucosamine-specific receptor which is postulated to trigger selective recycling of immature GlcNAc-bearing thyroglobulin molecules. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

hnRNP M: Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding; Receptor, misc.; Nucleolus; Spliceosome

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; spliceosome; extracellular matrix; nuclear matrix; membrane; integral to plasma membrane; nucleolus; paraspeckles

Molecular Function: protein domain specific binding; protein binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; alternative nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression