Mouse Ornithine Transcarbamylase (OTC) ELISA Kit | OTC elisa kit

Mouse Ornithine Transcarbamylase (OTC)(Sandwich ELISA) ELISA Kit

Cat. Num. AAA2709652

• Gene Names: Otc; Sf; spf; AI265390
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Ornithine Transcarbamylase (OTC); Mouse Ornithine Transcarbamylase (OTC)(Sandwich ELISA) ELISA Kit; ELISA Kit for Ornithine Transcarbamylase (OTC); OTC/Ornithine Transcarbamylase; OCTD; OCT; OTCase; Ornithine Transcarbamylase; Ornithine Carbamoyltransferase; OTC elisa kit

• Reactivity: Mouse
• Specificity: Reactive with Mouse Ornithine Transcarbamylase (OTC); This assay has high sensitivity and excellent specificity for detection of Ornithine Transcarbamylase (OTC). No significant cross-reactivity or interference between Ornithine Transcarbamylase (OTC) and analogues was observed.

• Applicable Applications for OTC elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Assay Type: Sandwich ELISA
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Detection Method: Double-antibody Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.113ng/mL
• Assay Length: 3 h
• Sample Size: 100ul (Please refer to the manual for the specific data.)
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ornithine Transcarbamylase (OTC) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ornithine Transcarbamylase (OTC) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C; 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C; 4. Aspirate and wash 3 times; 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 6. Aspirate and wash 5 times; 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C; 8. Add 50ul Stop Solution. Read at 450nm immediately.
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 6 (IGFBP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 6 (IGFBP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein 6 (IGFBP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Insulin Like Growth Factor Binding Protein 6 (IGFBP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: Store at 4 degree C for 1 month/ -20 degree C for long-term (within shelf life).; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for OTC elisa kit

Tumor immunity; Endocrinology

NCBI and Uniprot Product Information

• NCBI GI #: 6679184
• NCBI GeneID: 18416
• NCBI Accession #: NP_032795.1
• NCBI GenBank Nucleotide #: NM_008769.3
• UniProt Accession #: P11725
• Molecular Weight: 39,765 Da
• NCBI Official Full Name: ornithine carbamoyltransferase, mitochondrial
• NCBI Official Synonym Full Names: ornithine transcarbamylase
• NCBI Official Symbol: Otc
• NCBI Official Synonym Symbols: Sf; spf; AI265390
• NCBI Protein Information: ornithine carbamoyltransferase, mitochondrial; OTCase; sparse fur
• UniProt Protein Name: Ornithine carbamoyltransferase, mitochondrial
• UniProt Gene Name: Otc
• UniProt Synonym Gene Names: OTCase
• UniProt Entry Name: OTC_MOUSE

Uniprot Description

OTC: Defects in OTC are the cause of ornithine carbamoyltransferase deficiency (OTCD). OTCD is an X- linked disorder of the urea cycle which causes a form of hyperammonemia. Mutations with no residual enzyme activity are always expressed in hemizygote males by a very severe neonatal hyperammonemic coma that generally proves to be fatal. Heterozygous females are either asymptomatic or express orotic aciduria spontaneously or after protein intake. The disorder is treatable with supplemental dietary arginine and low protein diet. The arbitrary classification of patients into the 'neonatal' group (clinical hyperammonemia in the first few days of life) and 'late' onset (clinical presentation after the neonatal period) has been used to differentiate severe from mild forms. Belongs to the ATCase/OTCase family.

Protein type: Mitochondrial; Transferase; Amino Acid Metabolism - arginine and proline; EC 2.1.3.3

Cellular Component: mitochondrion; cytoplasm; mitochondrial inner membrane

Molecular Function: transferase activity; amino acid binding; carboxyl- or carbamoyltransferase activity; ornithine carbamoyltransferase activity; phospholipid binding; phosphate binding

Biological Process: amino acid metabolic process; citrulline biosynthetic process; arginine biosynthetic process; ornithine metabolic process; arginine biosynthetic process via ornithine; amino acid biosynthetic process; ornithine catabolic process; anion homeostasis; urea cycle