Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nuclear receptor coactivator 3 (NCOA3) Standard Curve.)
Mouse Nuclear Receptor Coactivator 3 (NCOA3) ELISA Kit | NCOA3 elisa kit
Mouse Nuclear Receptor Coactivator 3 (NCOA3) ELISA Kit
Gene Names
Ncoa3; Actr; Aib1; Rac3; Src3; pCip; Tram1; p/Cip; KAT13B; Tram-1; bHLHe42; AW321064; 2010305B15Rik
Reactivity
Mouse
Synonyms
Nuclear Receptor Coactivator 3 (NCOA3); Mouse Nuclear Receptor Coactivator 3 (NCOA3) ELISA Kit; NCOA3; RP5-1049G16.3; ACTR; AIB-1; AIB1; CAGH16; CTG26; KAT13B; MGC141848; RAC3; SRC-3; SRC3; TNRC14; TNRC16; TRAM-1; bHLHe42; pCIP; CBP-interacting protein; OTTHUMP00000031716; amplified in breast cancer-1; NCOA3 elisa kit
Reactivity
Mouse
Specificity
Mouse Nuclear receptor coactivator 3 (NCOA3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NCOA3. No significant cross-reactivity or interference between Mouse NCOA3 and analogues was observed.
Sequence Length
1398
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nuclear receptor coactivator 3 (NCOA3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nuclear receptor coactivator 3 (NCOA3) Standard Curve.)
Related Product Information for NCOA3 elisa kit
Description: This Mouse Nuclear receptor coactivator 3 (NCOA3) ELISA Kit employs a two-site sandwich ELISA to quantitate NCOA3 in samples. An antibody specific for NCOA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NCOA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCOA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCOA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, probably via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit (By similarity).
Background: Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, probably via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Nuclear receptor coactivator 3
NCBI Official Synonym Full Names
nuclear receptor coactivator 3
NCBI Official Symbol
Ncoa3
NCBI Official Synonym Symbols
Actr; Aib1; Rac3; Src3; pCip; Tram1; p/Cip; KAT13B; Tram-1; bHLHe42; AW321064; 2010305B15Rik
NCBI Protein Information
nuclear receptor coactivator 3
UniProt Protein Name
Nuclear receptor coactivator 3
Protein Family
UniProt Gene Name
Ncoa3
UniProt Synonym Gene Names
Aib1; Pcip; Rac3; Tram1; NCoA-3; AIB-1; p/CIP; pCIP; RAC-3; SRC-3; ACTR; TRAM-1
UniProt Entry Name
NCOA3_MOUSE
Research Articles on NCOA3
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Product Notes
The Mouse NCOA3 ncoa3 (Catalog #AAA9717410) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717410 ELISA Kit recognizes Mouse NCOA3. It is sometimes possible for the material contained within the vial of "Nuclear Receptor Coactivator 3 (NCOA3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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