Mouse Nuclear factor of activated T-cells, cytoplasmic 3, NFATC3 ELISA Kit | NFATC3 elisa kit

Mouse Nuclear factor of activated T-cells, cytoplasmic 3, NFATC3 ELISA Kit

Cat. Num. AAA2602702

• Gene Names: Nfatc3; NFAT4; NFATx; C80703; D8Ertd281e
• Reactivity: Mouse
• Synonyms: Nuclear factor of activated T-cells; cytoplasmic 3; NFATC3; Mouse Nuclear factor of activated T-cells; NFATC3 ELISA Kit; NFATC3 elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.

• Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant NFATC3 concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NFATC3 elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse NFATC3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 9087154
• NCBI GeneID: 18021
• UniProt Accession #: P97305; Q60896
• Molecular Weight: 115,451 Da
• NCBI Official Full Name: Nuclear factor of activated T-cells, cytoplasmic 3
• NCBI Official Synonym Full Names: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3
• NCBI Official Symbol: Nfatc3
• NCBI Official Synonym Symbols: NFAT4; NFATx; C80703; D8Ertd281e
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 3; NF-AT4; NF-ATc3; T-cell transcription factor NFAT4; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 3
• Protein Family: Nuclear factor of activated T-cells
• UniProt Gene Name: Nfatc3
• UniProt Synonym Gene Names: Nfat4; NF-ATc3; NFATc3; NF-AT4
• UniProt Entry Name: NFAC3_MOUSE

Uniprot Description

NFAT4: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells. Plays a role in the regulation of gene expression in T cells and immature thymocytes. Six spliced isoforms have been identified. Isoform 1 is predominantly expressed in thymus, peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.

Protein type: DNA-binding; Transcription factor

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; chromatin binding; transcription factor activity; transcription factor binding

Biological Process: cardiac muscle hypertrophy; transcription from RNA polymerase II promoter; transcription, DNA-dependent; thymus development; heart development; positive regulation of transcription, DNA-dependent; cellular respiration; myotube differentiation; muscle cell development; myotube cell development; skeletal muscle fiber development; patterning of blood vessels; calcineurin-NFAT signaling pathway; regulation of transcription, DNA-dependent; response to hypoxia; smooth muscle cell differentiation; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; T cell differentiation