Mouse Nodal Homolog (NODAL) ELISA Kit | NODAL elisa kit

Mouse Nodal Homolog (NODAL) ELISA Kit

Cat. Num. AAA2023875

• Gene Names: NODAL; HTX5
• Reactivity: Mouse
• Synonyms: Nodal Homolog (NODAL); Mouse Nodal Homolog (NODAL) ELISA Kit; NODAL elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nodal Homolog (NODAL).; No significant cross-reactivity or interference between Nodal Homolog (NODAL) and analogues was observed.
• Sequence Length: 347

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 6.1pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nodal Homolog (NODAL) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nodal Homolog (NODAL) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NODAL elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nodal Homolog (NODAL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nodal Homolog (NODAL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nodal Homolog (NODAL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nodal Homolog (NODAL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NODAL elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 222352098
• NCBI GeneID: 4838
• NCBI Accession #: NP_060525.3
• NCBI GenBank Nucleotide #: NM_018055.4
• UniProt Accession #: Q96S42; Q2M3A5; Q8N4V3
• Molecular Weight: 39,561 Da
• NCBI Official Full Name: nodal homolog
• NCBI Official Synonym Full Names: nodal growth differentiation factor
• NCBI Official Symbol: NODAL
• NCBI Official Synonym Symbols: HTX5
• NCBI Protein Information: nodal homolog
• UniProt Protein Name: Nodal homolog
• Protein Family: Nodal
• UniProt Gene Name: NODAL
• UniProt Entry Name: NODAL_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development. [provided by RefSeq, Jul 2008]

Uniprot Description

NODAL: Essential for mesoderm formation and axial patterning during embryonic development. Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5). A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide; Cytokine; Motility/polarity/chemotaxis; Cell development/differentiation

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: extracellular space

Molecular Function: morphogen activity; growth factor activity; cytokine activity; receptor agonist activity; transforming growth factor beta receptor binding

Biological Process: embryonic placenta development; mesendoderm development; positive regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; axial mesodermal cell fate specification; embryonic pattern specification; floor plate morphogenesis; positive regulation of activin receptor signaling pathway; vasculature development; regulation of gastrulation; positive regulation of cell-cell adhesion; trophectodermal cellular morphogenesis; heart looping; cell migration involved in gastrulation; placenta development; maternal placenta development; stem cell maintenance; embryonic process involved in female pregnancy; digestive tract morphogenesis; embryonic cranial skeleton morphogenesis; liver development; formation of anatomical boundary; neural fold formation; positive regulation of angiogenesis; repression of premature neural plate formation; maternal process involved in parturition; polarity specification of proximal/distal axis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; brain development; positive regulation of epithelial cell proliferation; growth

Disease: Heterotaxy, Visceral, 5, Autosomal