Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Neuropeptide Y (NPY) Standard Curve.)
Mouse Neuropeptide Y (NPY) ELISA Kit | NPY elisa kit
Mouse Neuropeptide Y (NPY) ELISA Kit
Gene Names
Npy; 0710005A05Rik
Reactivity
Mouse
Synonyms
Neuropeptide Y (NPY); Mouse Neuropeptide Y (NPY) ELISA Kit; PYY4; OTTHUMP00000201946; OTTHUMP00000201947; NPY elisa kit
Reactivity
Mouse
Specificity
Mouse Neuropeptide Y (NPY) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NPY. No significant cross-reactivity or interference between Mouse NPY and analogues was observed.
Sequence Length
97
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Neuropeptide Y (NPY) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Neuropeptide Y (NPY) Standard Curve.)
Related Product Information for NPY elisa kit
Description: This Mouse Neuropeptide Y (NPY) ELISA Kit employs a two-site sandwich ELISA to quantitate NPY in samples. An antibody specific for NPY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NPY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPY bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter found in the brain and autonomic nervous system.NPY is a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases.
Background: Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter found in the brain and autonomic nervous system.NPY is a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pro-neuropeptide Y preproprotein
NCBI Official Synonym Full Names
neuropeptide Y
NCBI Official Symbol
Npy
NCBI Official Synonym Symbols
0710005A05Rik
NCBI Protein Information
pro-neuropeptide Y
UniProt Protein Name
Pro-neuropeptide Y
Protein Family
UniProt Gene Name
Npy
UniProt Synonym Gene Names
NPY; CPON
NCBI Description
This gene encodes a neuropeptide that plays a pivotal role in many physiological functions such as food intake, energy homeostasis, circadian rhythm, and cognition. The encoded protein precursor undergoes proteolytic processing to generate the biologically active peptide. Mice lacking the encoded protein exhibit mild seizures occasionally and become hyperphagic following food deprivation. A deficiency of the encoded protein partially prevents mice lacking leptin from becoming obese. [provided by RefSeq, Oct 2015]
Uniprot Description
NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Research Articles on NPY
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Product Notes
The Mouse NPY npy (Catalog #AAA9718166) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718166 ELISA Kit recognizes Mouse NPY. It is sometimes possible for the material contained within the vial of "Neuropeptide Y (NPY), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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