Mouse neuregulin-4 (NRG4) ELISA Kit | NRG4 elisa kit

Mouse neuregulin-4, NRG4 ELISA Kit

Cat. Num. AAA910811

• Gene Names: Nrg4; AI552600
• Reactivity: Mouse
• Synonyms: neuregulin-4 (NRG4); Mouse neuregulin-4; NRG4 ELISA Kit; Mouse neuregulin-4 (NRG4) ELISA kit; DKFZp779N0541; DKFZp779N1944; HRG4; heregulin 4; NRG4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse NRG4. No significant cross-reactivity or interference between mouse NRG4 and analogues was observed.
• Sequence Length: 115

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 3.12 ng/ml-200 ng/ml
• Sensitivity: < 0.78 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NRG4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NRG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NRG4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRG4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRG4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 14030777
• NCBI GeneID: 83961
• NCBI Accession #: NP_114391.1
• NCBI GenBank Nucleotide #: NM_032002.2
• UniProt Accession #: Q9WTX4
• Molecular Weight: 12,744 Da
• NCBI Official Full Name: pro-neuregulin-4, membrane-bound isoform
• NCBI Official Synonym Full Names: neuregulin 4
• NCBI Official Symbol: Nrg4
• NCBI Official Synonym Symbols: AI552600
• NCBI Protein Information: pro-neuregulin-4, membrane-bound isoform; pro-NRG4
• UniProt Protein Name: Pro-neuregulin-4, membrane-bound isoform
• Protein Family: Pro-neuregulin
• UniProt Gene Name: Nrg4
• UniProt Synonym Gene Names: Pro-NRG4; NRG-4
• UniProt Entry Name: NRG4_MOUSE

Uniprot Description

NRG4: Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors. Belongs to the neuregulin family.

Protein type: Membrane protein, integral

Cellular Component: extracellular space; membrane; integral to membrane; plasma membrane; extracellular region; intracellular

Molecular Function: growth factor activity; receptor binding

Biological Process: organ development