Rabbit anti-Human CHST6 Polyclonal Antibody | anti-CHST6 antibody

CHST6, CT (CHST6, Carbohydrate sulfotransferase 6, Corneal N-acetylglucosamine-6-O-sulfotransferase, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-beta, N-acetylglucosamine 6-O-sulfotransferase 5) (FITC)

Cat. Num. AAA6285978

• Gene Names: CHST6; MCDC1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CHST6; Polyclonal Antibody; CT (CHST6; Carbohydrate sulfotransferase 6; Corneal N-acetylglucosamine-6-O-sulfotransferase; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-beta; N-acetylglucosamine 6-O-sulfotransferase 5) (FITC); N-acetylglucosamine 6-O-sulfotransferase 5; anti-CHST6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CHST6 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CHST6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-333 amino acids from the C-terminal region of human CHST6.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CHST6 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CHST6 antibody detected the CHST6 protein (arrow).)

Immunohistochemistry (IHC)

(CHST6 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CHST6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CHST6 antibody

The protein encoded by this gene is an enzyme that catalyzes the transfer of a sulfate group to the GlcNAc residues of keratan. Keratan sulfate helps maintain corneal transparency. Defects in this gene are a cause of macular corneal dystrophy (MCD).

Product Categories/Family for anti-CHST6 antibody

Antibodies; Enzymes; Sulfotransferase

References

Liu, Z., et al. Cornea 29(8):883-888(2010). Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010). Dang, X., et al. Mol. Vis. 15, 700-705 (2009): Birgani, S.A., et al. Mol. Vis. 15, 373-377 (2009): Sultana, A., et al. Mol. Vis. 15, 319-325 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 11055976
• NCBI GeneID: 4166
• NCBI Accession #: NP_067628.1
• NCBI GenBank Nucleotide #: NM_021615.4
• UniProt Accession #: Q9GZX3; D3DUK3
• Molecular Weight: 44,099 Da
• NCBI Official Full Name: carbohydrate sulfotransferase 6
• NCBI Official Synonym Full Names: carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 6
• NCBI Official Symbol: CHST6
• NCBI Official Synonym Symbols: MCDC1
• NCBI Protein Information: carbohydrate sulfotransferase 6; gn6st-5; hCGn6ST; GST4-beta; C-GlcNAc6ST; glcNAc6ST-5; N-acetylglucosamine 6-O-sulfotransferase 5; corneal N-acetylglucosamine 6-sulfotransferase; corneal N-acetylglucosamine-6-O-sulfotransferase; galactose/N-acetylglucosa
• UniProt Protein Name: Carbohydrate sulfotransferase 6
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: CHST6
• UniProt Synonym Gene Names: C-GlcNAc6ST; hCGn6ST; GST4-beta; GlcNAc6ST-5; Gn6st-5
• UniProt Entry Name: CHST6_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that catalyzes the transfer of a sulfate group to the GlcNAc residues of keratan. Keratan sulfate helps maintain corneal transparency. Defects in this gene are a cause of macular corneal dystrophy (MCD). [provided by RefSeq, Jan 2010]

Uniprot Description

CHST6: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N- acetyllactosamine structures. Defects in CHST6 are the cause of macular corneal dystrophy (MCD). MCD is an autosomal recessive disease characterized by corneal opacities. Onset occurs in the first decade, usually between ages 5 and 9. The disorder is progressive. Minute, gray, punctate opacities develop. Corneal sensitivity is usually reduced. Painful attacks with photophobia, foreign body sensations, and recurrent erosions occur in most patients. There are different types of MCD: MCD type I, in which there is a virtual absence of sulfated keratan sulfate (KS) in the serum and cornea, as determined by KS-specific antibodies; and MCD type II, in which the normal sulfated KS-antibody response is present in cornea and serum. MCD type I patients usually have a homozygous missense mutation, while MCD type II patients show a large deletion and replacement in the upstream region of CHST6. The only missense mutation for type II is Cys-50, which is heterozygous with a replacement in the upstream region on the other allele of CHST6. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Protein type: Transferase; Membrane protein, integral; Glycan Metabolism - keratan sulfate biosynthesis; EC 2.8.2.-

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: Golgi membrane; Golgi apparatus; integral to membrane

Molecular Function: N-acetylglucosamine 6-O-sulfotransferase activity

Biological Process: keratan sulfate metabolic process; sulfur metabolic process; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; carbohydrate metabolic process; N-acetylglucosamine metabolic process; pathogenesis

Disease: Macular Dystrophy, Corneal, 1