Typical Testing Data/Standard Curve (for reference only)
Mouse Myogenin (MYOG) ELISA Kit | MYOG elisa kit
Mouse Myogenin (MYOG) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to myogenin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to myogenin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain myogenin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of myogenin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008]
Uniprot Description
myogenin: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 1q31-q41
Cellular Component: nucleoplasm; transcription factor complex; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; chromatin DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity
Biological Process: skeletal muscle development; ossification; muscle cell fate commitment; skeletal muscle fiber development; response to electrical stimulus involved in regulation of muscle adaptation; response to denervation involved in regulation of muscle adaptation; response to muscle activity involved in regulation of muscle adaptation; cell cycle; mRNA transcription from RNA polymerase II promoter; positive regulation of myoblast differentiation; negative regulation of cell proliferation; muscle cell differentiation; positive regulation of muscle atrophy; striated muscle atrophy; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of satellite cell proliferation
Research Articles on MYOG
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Product Notes
The Mouse MYOG myog (Catalog #AAA2021050) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2021050 ELISA Kit recognizes Mouse MYOG. It is sometimes possible for the material contained within the vial of "Myogenin (MYOG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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