Mouse mucin-5 subtype B, MUC5B ELISA Kit | MUC5B elisa kit

Mouse mucin-5 subtype B, MUC5B ELISA Kit

Cat. Num. AAA705579

• Gene Names: MUC5B; MG1; MUC5; MUC9; MUC-5B
• Reactivity: Mouse
• Synonyms: mucin-5 subtype B; MUC5B; Mouse mucin-5 subtype B; MUC5B ELISA Kit; MUC5B elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse MUC5B . No significant cross-reactivity or interference between mouse MUC5B and analogues was observed.
• Sequence Length: 5762

• Samples: Serum, plasma, cell culture supernates and tissue homogenates
• Assay Type: Sandwich
• Detection Range: 1.25 ng/ml -80 ng/ml.
• Sensitivity: The minimum detectable dose of mouse MUC5B is typically less than 0.31 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MUC5B elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MUC5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC5B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC5B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC5B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 301172750
• NCBI GeneID: 727897
• NCBI Accession #: NP_002449.2
• NCBI GenBank Nucleotide #: NM_002458.2
• UniProt Accession #: Q9HC84; O00447; O00573; O14985; O15494; O95291; O95451; Q14881; Q7M4S5; Q99552; Q9UE28
• Molecular Weight: 596,340 Da
• NCBI Official Full Name: mucin-5B
• NCBI Official Synonym Full Names: mucin 5B, oligomeric mucus/gel-forming
• NCBI Official Symbol: MUC5B
• NCBI Official Synonym Symbols: MG1; MUC5; MUC9; MUC-5B
• NCBI Protein Information: mucin-5B; cervical mucin MUC5B; sublingual gland mucin; mucin 5, subtype B, tracheobronchial; high molecular weight salivary mucin MG1
• UniProt Protein Name: Mucin-5B
• Protein Family: Mucin
• UniProt Gene Name: MUC5B
• UniProt Synonym Gene Names: MUC5; MUC-5B
• UniProt Entry Name: MUC5B_HUMAN

NCBI Description

This gene encodes a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases, including sinus mucosa of chronic rhinosinusitis (CRS), CRS with nasal polyposis, chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease, and it may be involved in the pathogenesis of these diseases. [provided by RefSeq, Jul 2010]

Uniprot Description

Function: Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.