Mouse Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) ELISA Kit | MHCDRb1 elisa kit

Mouse Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) ELISA Kit

Cat. Num. AAA2021965

• Gene Names: HLA-DRB1; SS1; DRB1; DRw10; HLA-DRB; HLA-DR1B
• Reactivity: Mouse
• Synonyms: Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1); Mouse Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) ELISA Kit; HLA-DRB1; HLA-DR1B; HLADRb1; HLA Class II Histocompatibility Antigen; DRB1-9 Beta Chain; HLA class II histocompatibility antigen; DRB1-15 beta chain; MHCDRb1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1).; No significant cross-reactivity or interference between Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) and analogues was observed.
• Sequence Length: 266

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 39.06-2500pg/mL
• Sensitivity: < 12pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MHCDRb1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MHCDRb1 elisa kit

Tumor immunity; Infection immunity; Immune molecule; Immunodeficiency; Autoimmunity

NCBI and Uniprot Product Information

• NCBI GI #: 345461076
• NCBI GeneID: 3123
• NCBI Accession #: NP_001230894.1
• NCBI GenBank Nucleotide #: NM_001243965.1
• UniProt Accession #: P01911; Q29790; Q29975; Q30142; Q30166; Q32MY7; Q56FN9; Q5Y7B0; Q5Y7B9; P01912; P04229; P20039; Q29974; Q30134; Q30167; Q5Y7A7; Q95IE3; Q9GIY3
• Molecular Weight: 29,966 Da
• NCBI Official Full Name: major histocompatibility complex, class II, DR beta 1
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DR beta 1
• NCBI Official Symbol: HLA-DRB1
• NCBI Official Synonym Symbols: SS1; DRB1; DRw10; HLA-DRB; HLA-DR1B
• NCBI Protein Information: major histocompatibility complex, class II, DR beta 1
• UniProt Protein Name: HLA class II histocompatibility antigen, DRB1-15 beta chain
• UniProt Gene Name: HLA-DRB1
• UniProt Synonym Gene Names: HLA-DRB2
• UniProt Entry Name: 2B1F_HUMAN

NCBI Description

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DRB1 iso2: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route; where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules; and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments; exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides; autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs; other cells of the gastrointestinal tract; such as epithelial cells; express MHC class II molecules and CD74 and act as APCs; which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen; three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs; CD74 undergoes a sequential degradation by various proteases; including CTSS and CTSL; leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells; the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules; increased acidification produces increased proteolysis and efficient peptide loading. Genetic variation in HLA-DRB1 is a cause of susceptibility to sarcoidosis type 1 (SS1). Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; cell surface; membrane; late endosome membrane; integral to plasma membrane; lysosomal membrane; plasma membrane; trans-Golgi network membrane; external side of plasma membrane; MHC class II protein complex

Molecular Function: MHC class II receptor activity; peptide antigen binding

Biological Process: T-helper 1 type immune response; detection of bacterium; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; immunoglobulin production during immune response; T cell receptor signaling pathway; polysaccharide assembly with MHC class II protein complex; humoral immune response mediated by circulating immunoglobulin; inflammatory response to antigenic stimulus; negative regulation of T cell proliferation; peptide antigen assembly with MHC class II protein complex; regulation of interleukin-4 production; negative regulation of interferon-gamma production; T cell costimulation; immune response; protein tetramerization