Mouse Lysozyme (LZM) ELISA Kit | LZM elisa kit

Mouse Lysozyme (LZM) ELISA Kit

Cat. Num. AAA452408

• Gene Names: Lyz2; Lys; Lzm; Lzp; Lysm; Lyzs; Lyzf2; Lzm-s1; AI326280
• Reactivity: Mouse
• Synonyms: Lysozyme (LZM); Mouse Lysozyme (LZM) ELISA Kit; LYZ; Renal Amyloidosis; N-Acetylmuramide Glycanhydrolase; Muramidase; 1; 4-beta-N-acetylmuramidase C; LZM elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of LZM. No significant cross-reactivity or interference between LZM and analogues was observed.
• Sequence Length: 148

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.066ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LZM were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LZM were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LZM elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LZM in mouse serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LZM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LZM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LZM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LZM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8393739
• NCBI GeneID: 17105
• NCBI Accession #: NP_059068.1
• NCBI GenBank Nucleotide #: NM_017372.3
• UniProt Accession #: P08905; Q3TXG2; Q3U5Q2; Q3U690; Q8VE78
• Molecular Weight: 16,689 Da
• NCBI Official Full Name: lysozyme C-2
• NCBI Official Synonym Full Names: lysozyme 2
• NCBI Official Symbol: Lyz2
• NCBI Official Synonym Symbols: Lys; Lzm; Lzp; Lysm; Lyzs; Lyzf2; Lzm-s1; AI326280
• NCBI Protein Information: lysozyme C-2
• UniProt Protein Name: Lysozyme C-2
• UniProt Gene Name: Lyz2
• UniProt Synonym Gene Names: Lyz; Lyzs

Uniprot Description

Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Lyz2 is active against a range of Gram-positive and Gram-negative bacteria. More effective than Lyz1 in killing Gram-negative bacteria. Lyz1 and Lyz2 are equally effective in killing Gram-positive bacteria.