Mouse LOX-1/OLR1 ELISA Kit | OLR1 elisa kit

Mouse LOX-1/OLR1 PicoKine ELISA Kit

Cat. Num. AAA175926

• Gene Names: Olr1; LOX-1; SR-EI; Scare1
• Reactivity: Mouse
• Synonyms: LOX-1/OLR1; Mouse LOX-1/OLR1 PicoKine ELISA Kit; Oxidized low-density lipoprotein receptor 1; oxidized low density lipoprotein (lectin-like) receptor 1; C-type lectin domain family 8 member A; CLEC8A; hLOX-1; Lectin-like oxidized LDL receptor 1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1; LOX-1; LOXIN; Olr1; OLR1_HUMAN; Ox-LDL receptor 1; Oxidized low density lipoprotein (lectin like) receptor 1; Oxidized low density lipoprotein receptor 1 soluble form; SCARE1; Scavenger receptor class E member 1; SLOX1; OLR1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse LOX-1
• Sequence Length: 363

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 1pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse LOX-1/OLR1 PicoKine ELISA Kit standard curve )

Related Product Information for OLR1 elisa kit

Principle of the assay: mouse LOX-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for LOX-1 has been precoated onto 96-well plates. Standards(NSO, R60-I363) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LOX-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse LOX-1 amount of sample captured in plate.
Background: Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) also known as lectin-type oxidized LDL receptor 1 (LOX-1) is a protein that in humans is encoded by the OLR1 gene. It belongs to the C-type lectin superfamily. The LOX1 gene is mapped to to 12p13-p12. The protein has got the amino acids of 363. It can be detected in various tissues, highly expressed in placenta. This protein may be involved in the regulation of Fas-induced apoptosis and play a role as a scavenger receptor. The standards of this kit are recombinant mouse LOX-1 (R60-I363), with molecular weight of 36.3kDa.

NCBI and Uniprot Product Information

• NCBI GI #: 341941209
• NCBI GeneID: 108078
• UniProt Accession #: Q9EQ09; Q3U3M1
• Molecular Weight: 41,643 Da
• NCBI Official Full Name: Oxidized low-density lipoprotein receptor 1
• NCBI Official Synonym Full Names: oxidized low density lipoprotein (lectin-like) receptor 1
• NCBI Official Symbol: Olr1
• NCBI Official Synonym Symbols: LOX-1; SR-EI; Scare1
• NCBI Protein Information: oxidized low-density lipoprotein receptor 1; ox-LDL receptor 1; lectin-like oxLDL receptor 1; lectin-like oxidized LDL receptor 1; lectin-type oxidized LDL receptor 1; oxidised low density lipoprotein (lectin-like) receptor 1
• UniProt Protein Name: Oxidized low-density lipoprotein receptor 1
• Protein Family: Oxidized low-density lipoprotein receptor
• UniProt Gene Name: Olr1
• UniProt Synonym Gene Names: Lox1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1
• UniProt Entry Name: OLR1_MOUSE

Uniprot Description

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria ().