Mouse LHRH ELISA Kit | LHRH elisa kit
Mouse LHRH (Luteinizing Hormone-Releasing Hormone) ELISA Kit
Gene Names
GNRH1; GNRH; LHRH
Reactivity
Mouse
Synonyms
LHRH; Mouse LHRH (Luteinizing Hormone-Releasing Hormone) ELISA Kit; LHRH elisa kit
Reactivity
Mouse
Specificity
This kit recognizes natural and recombinant Mouse LHRH. No significant cross-reactivity or interference between Mouse LHRH and analogues was observed.
Sequence Length
67
Samples
Serum, Plasma, Biological Fluids
Assay Type
Competitive
Detection Range
78.125-5000pg/mL
Sensitivity
Min: 46.875pg/mL; Max: 5000pg/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LHRH elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse LHRH concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LHRH. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LHRH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LHRH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of LHRH. You can calculate the concentration of LHRH in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LHRH. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LHRH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LHRH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of LHRH. You can calculate the concentration of LHRH in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
7,573 Da
NCBI Official Full Name
luteinizing hormone-releasing hormone, partial
NCBI Official Symbol
GNRH1
NCBI Official Synonym Symbols
GNRH; LHRH
NCBI Protein Information
progonadoliberin-1; luteinizing hormone-releasing hormone; progonadoliberin I; progonadoliberin-1 preproprotein
UniProt Protein Name
Progonadoliberin-1
UniProt Gene Name
GNRH1
UniProt Synonym Gene Names
GNRH; LHRH; GnRH-I; LH-RH I
UniProt Entry Name
GON1_MACMU
Uniprot Description
Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones.
