Mouse IQ Motif Containing GTPase Activating Protein 2 (IQGAP2) ELISA Kit | IQGAP2 elisa kit

Mouse IQ Motif Containing GTPase Activating Protein 2 (IQGAP2) ELISA Kit

Cat. Num. AAA2020889

• Reactivity: Mouse
• Synonyms: IQ Motif Containing GTPase Activating Protein 2 (IQGAP2); Mouse IQ Motif Containing GTPase Activating Protein 2 (IQGAP2) ELISA Kit; IQGAP2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of IQ Motif Containing GTPase Activating Protein 2 (IQGAP2).; No significant cross-reactivity or interference between IQ Motif Containing GTPase Activating Protein 2 (IQGAP2) and analogues was observed.
• Sequence Length: 1575

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.062ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IQ Motif Containing GTPase Activating Protein 2 (IQGAP2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IQ Motif Containing GTPase Activating Protein 2 (IQGAP2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IQGAP2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IQ Motif Containing GTPase Activating Protein 2 (IQGAP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IQ Motif Containing GTPase Activating Protein 2 (IQGAP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IQ Motif Containing GTPase Activating Protein 2 (IQGAP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IQ Motif Containing GTPase Activating Protein 2 (IQGAP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IQGAP2 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 119616183
• NCBI GeneID: 10788
• UniProt Accession #: Q13576; A8K4V1; B7Z8A4; J3KR91
• Molecular Weight: 122,806 Da
• NCBI Official Full Name: IQ motif containing GTPase activating protein 2, isoform CRA_b
• NCBI Official Synonym Full Names: IQ motif containing GTPase activating protein 2
• NCBI Official Symbol: IQGAP2
• NCBI Protein Information: ras GTPase-activating-like protein IQGAP2
• UniProt Protein Name: Ras GTPase-activating-like protein IQGAP2
• Protein Family: Ras GTPase-activating-like protein
• UniProt Gene Name: IQGAP2
• UniProt Entry Name: IQGA2_HUMAN

NCBI Description

This gene encodes a member of the IQGAP family. The protein contains three IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. [provided by RefSeq, Jul 2008]

Uniprot Description

IQGAP2: Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin. Isoform 2 expression is enhanced in testis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein regulator, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: filamentous actin; microtubule; cell surface; microvillus; lamellipodium; cytoplasm; filopodium; actin cytoskeleton

Molecular Function: calmodulin binding; actin filament binding; phosphatidylinositol-3,4,5-triphosphate binding; Rho GTPase binding; Rac GTPase binding; actin binding; GTPase inhibitor activity

Biological Process: small GTPase mediated signal transduction; negative regulation of catalytic activity; signal transduction