Mouse Interleukin 12A (IL12A) ELISA Kit | IL12A elisa kit

Mouse Interleukin 12A (IL12A) Mini Samples ELISA Kit

Cat. Num. AAA2089486

• Gene Names: Il12a; p35; Ll12a; Il-12a; IL-12p35
• Reactivity: Mouse
• Synonyms: Interleukin 12A (IL12A); Mouse Interleukin 12A (IL12A) Mini Samples ELISA Kit; p35; IL12-A; CLMF1; NFSK; NKSF1; Natural Killer Cell Stimulatory Factor 1; Cytotoxic Lymphocyte Maturation Factor 1; NF Cell Stimulatory Factor Chain 1; IL12A elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 12A (IL12A).; No significant cross-reactivity or interference between Mini Samples Interleukin 12A (IL12A) and analogues was observed.
• Sequence Length: 236

• Assay Type: Double-antibody Sandwich
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 12A (IL12A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 12A (IL12A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Related Product Information for IL12A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 12A (IL12A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Interleukin 12A (IL12A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Interleukin 12A (IL12A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Interleukin 12A (IL12A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL12A elisa kit

Cytokine; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 226874945
• NCBI GeneID: 16159
• NCBI Accession #: NP_001152896.1
• NCBI GenBank Nucleotide #: NM_001159424.2
• UniProt Accession #: P43431
• Molecular Weight: 24,179 Da
• NCBI Official Full Name: interleukin-12 subunit alpha isoform 1
• NCBI Official Synonym Full Names: interleukin 12a
• NCBI Official Symbol: Il12a
• NCBI Official Synonym Symbols: p35; Ll12a; Il-12a; IL-12p35
• NCBI Protein Information: interleukin-12 subunit alpha
• UniProt Protein Name: Interleukin-12 subunit alpha
• Protein Family: Interleukin
• UniProt Gene Name: Il12a
• UniProt Synonym Gene Names: IL-12A; CLMF p35

Uniprot Description

IL12A: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Belongs to the IL-6 superfamily.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3 E1|3 31.92 cM

Cellular Component: cytoplasm; extracellular space; interleukin-12 complex

Molecular Function: cytokine activity; growth factor activity; interleukin-12 beta subunit binding; interleukin-12 receptor binding; interleukin-27 binding; protein heterodimerization activity

Biological Process: cell cycle arrest; cell migration; defense response to protozoan; negative regulation of interleukin-17 production; negative regulation of smooth muscle cell proliferation; positive regulation of cell adhesion; positive regulation of interferon-gamma production; positive regulation of lymphocyte proliferation; positive regulation of mononuclear cell proliferation; positive regulation of natural killer cell activation; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; positive regulation of NK T cell activation; positive regulation of T cell differentiation; positive regulation of T cell mediated cytotoxicity; positive regulation of T cell proliferation; positive regulation of tyrosine phosphorylation of Stat4 protein; response to lipopolysaccharide; response to UV-B