Mouse Interleukin 12 (IL12) ELISA Kit | IL12 elisa kit

Mouse Interleukin 12 (IL12) ELISA Kit

Cat. Num. AAA2701247

• Gene Names: IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
• Reactivity: Mouse
• Synonyms: Interleukin 12 (IL12); Mouse Interleukin 12 (IL12) ELISA Kit; IL12 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 12 (IL12).; No significant cross-reactivity or interference between Interleukin 12 (IL12) and analogues was observed.
• Sequence Length: 253

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 7.8-500pg/mL
• Sensitivity: < 2.8pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 12 (IL12) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 12 (IL12) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL12 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12 (IL12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12 (IL12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12 (IL12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 12 (IL12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4323579
• NCBI GeneID: 3592
• Molecular Weight: 24,874 Da
• NCBI Official Full Name: interleukin 12
• NCBI Official Synonym Full Names: interleukin 12A
• NCBI Official Symbol: IL12A
• NCBI Official Synonym Symbols: P35; CLMF; NFSK; NKSF1; IL-12A
• NCBI Protein Information: interleukin-12 subunit alpha
• UniProt Protein Name: Interleukin-12 subunit alpha
• UniProt Gene Name: IL12A
• UniProt Synonym Gene Names: NKSF1; IL-12A; CLMF p35; NKSF1

NCBI Description

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Uniprot Description

IL12A: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Belongs to the IL-6 superfamily.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q25.33

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; interleukin-12 complex

Molecular Function: cytokine activity; growth factor activity; interleukin-12 beta subunit binding; interleukin-12 receptor binding; interleukin-27 binding; protein binding; protein heterodimerization activity

Biological Process: cell cycle arrest; cell migration; defense response to Gram-positive bacterium; defense response to protozoan; immune response; negative regulation of interleukin-17 production; negative regulation of smooth muscle cell proliferation; positive regulation of cell adhesion; positive regulation of interferon-gamma production; positive regulation of lymphocyte proliferation; positive regulation of mononuclear cell proliferation; positive regulation of natural killer cell activation; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; positive regulation of NK T cell activation; positive regulation of T cell differentiation; positive regulation of T cell mediated cytotoxicity; positive regulation of T cell proliferation; positive regulation of tyrosine phosphorylation of STAT protein; response to lipopolysaccharide; response to UV-B; response to virus