Typical Testing Data/Standard Curve (for reference only)
Mouse Inter-alpha-trypsin inhibitor heavy chain H1 (ITIH1) ELISA Kit | ITIH1 elisa kit
Mouse Inter-alpha-trypsin inhibitor heavy chain H1 (ITIH1) ELISA Kit
Gene Names
ITIH1; H1P; ITIH; SHAP; IATIH; IGHEP1; ITI-HC1
Reactivity
Mouse
Synonyms
Inter-alpha-trypsin inhibitor heavy chain H1 (ITIH1); Mouse Inter-alpha-trypsin inhibitor heavy chain H1 (ITIH1) ELISA Kit; ITIH1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of ITIH1. No significant cross-reactivity or interference between ITIH1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ITIH1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ITIH1 elisa kit
Intended Uses: This ITIH1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ITIH1. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: ITIH1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ITIH1 antibody and an ITIH1-HRP conjugate. The assay sample and buffer are incubated together with ITIH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ITIH1 concentration since ITIH1 from samples and ITIH1-HRP conjugate compete for the anti-ITIH1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ITIH1 from the sample, fewer sites are left to bind ITIH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITIH1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ITIH1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ITIH1 antibody and an ITIH1-HRP conjugate. The assay sample and buffer are incubated together with ITIH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ITIH1 concentration since ITIH1 from samples and ITIH1-HRP conjugate compete for the anti-ITIH1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ITIH1 from the sample, fewer sites are left to bind ITIH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITIH1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ITIH1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,495 Da
NCBI Official Full Name
inter-alpha-trypsin inhibitor heavy chain H1 isoform a
NCBI Official Synonym Full Names
inter-alpha-trypsin inhibitor heavy chain 1
NCBI Official Symbol
ITIH1
NCBI Official Synonym Symbols
H1P; ITIH; SHAP; IATIH; IGHEP1; ITI-HC1
NCBI Protein Information
inter-alpha-trypsin inhibitor heavy chain H1; ITI heavy chain H1; inter-alpha (globulin) inhibitor H1; inter-alpha-inhibitor heavy chain 1; serum-derived hyaluronan-associated protein; inter-alpha (globulin) inhibitor, H1 polypeptide; inter-alpha-trypsin inhibitor complex component III
UniProt Protein Name
Inter-alpha-trypsin inhibitor heavy chain H1
Protein Family
UniProt Gene Name
ITIH1
UniProt Synonym Gene Names
IGHEP1; ITI heavy chain H1; ITI-HC1; Inter-alpha-inhibitor heavy chain 1; SHAP
UniProt Entry Name
ITIH1_HUMAN
NCBI Description
The protein encoded by this gene is the heavy chain of a serine protease inhibitor that may serve to carry hyaluronan in plasma. This gene is part of a cluster of similar genes on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Uniprot Description
ITIH1: May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Belongs to the ITIH family.
Protein type: Inhibitor; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 3p21.1
Cellular Component: extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; calcium ion binding
Biological Process: hyaluronan metabolic process
Research Articles on ITIH1
Similar Products
Product Notes
The Mouse ITIH1 itih1 (Catalog #AAA7212790) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7212790 ELISA Kit recognizes Mouse ITIH1. It is sometimes possible for the material contained within the vial of "Inter-alpha-trypsin inhibitor heavy chain H1 (ITIH1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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