Mouse Inhibin A (INHA) ELISA Kit | INHA elisa kit

Mouse Inhibin A (INHA) ELISA Kit

Cat. Num. AAA2086971

• Reactivity: Mouse
• Synonyms: Inhibin A (INHA); Mouse Inhibin A (INHA) ELISA Kit; INH-A; INHA elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of IgG. No significant cross-reactivity or interference between IgG and analogues was observed.
• Sequence Length: 366

• Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
• Assay Type: Quantitative Competitive
• Detection Range: 1.23-100ug/mL
• Sensitivity: 0.53ug/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IgG were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IgG were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for INHA elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of IgG in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to IgG has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled IgG and unlabeled IgG (Standards or samples) with the pre-coated antibody specific to IgG. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of IgG in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of IgG in the sample.

Product Categories/Family for INHA elisa kit

Endocrinology; Reproductive science; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 4504697
• NCBI GeneID: 3623
• NCBI Accession #: NP_002182.1
• NCBI GenBank Nucleotide #: NM_002191.3
• UniProt Accession #: P05111; A8K8H5
• Molecular Weight: 39,670 Da
• NCBI Official Full Name: inhibin alpha chain preproprotein
• NCBI Official Synonym Full Names: inhibin alpha subunit
• NCBI Official Symbol: INHA
• NCBI Protein Information: inhibin alpha chain
• UniProt Protein Name: Inhibin alpha chain
• Protein Family: Inhibin
• UniProt Gene Name: INHA

NCBI Description

This gene encodes a member of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate multiple peptide products, including the alpha subunit of the inhibin A and B protein complexes. These complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion. Mutations in this gene may be associated with male infertility and premature ovarian failure in female human patients. [provided by RefSeq, Aug 2016]

Uniprot Description

INHA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: extracellular region

Molecular Function: cytokine activity; growth factor activity; hormone activity; protein binding; receptor binding; transforming growth factor beta receptor binding

Biological Process: cell cycle arrest; cell development; cell differentiation; cell surface receptor linked signal transduction; cell-cell signaling; hemoglobin biosynthetic process; negative regulation of B cell differentiation; negative regulation of cell cycle; negative regulation of interferon-gamma biosynthetic process; negative regulation of macrophage differentiation; negative regulation of phosphorylation; positive regulation of follicle-stimulating hormone secretion; regulation of apoptosis; regulation of cell cycle; regulation of cell proliferation; regulation of MAPKKK cascade; response to external stimulus; signal transduction; skeletal development