Mouse IGFBP1 ELISA Kit | IGFBP1 elisa kit

Mouse IGFBP1 ELISA Kit

Cat. Num. AAA824717

• Reactivity: Mouse
• Applications: ELISA
• Synonyms: IGFBP1; Mouse IGFBP1 ELISA Kit; IBP1; Insulin-like growth factor-binding protein 1; IBP-1; IGF-binding protein 1; IGFBP-1; Placental protein 12; PP12; IGFBP1 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse IGFBP1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse IGFBP1 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
• Sequence Length: 272

• Applicable Applications for IGFBP1 elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma.
• Assay Type: Sandwich
• Sensitivity: The minimum detectable dose of Mouse IGFBP1 is typically less than 3 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for IGFBP1 elisa kit

Principle of the Assay: The Mouse IGFBP1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP1 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse IGFBP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IGFBP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7.

NCBI and Uniprot Product Information

• NCBI GI #: 160358847
• NCBI GeneID: 16006
• NCBI Accession #: NP_032367.3
• NCBI GenBank Nucleotide #: NM_008341.4
• UniProt Accession #: P47876; Q5SVY8; Q61732
• Molecular Weight: 29,570 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 1
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 1
• NCBI Official Symbol: Igfbp1
• NCBI Protein Information: insulin-like growth factor-binding protein 1; IBP-1; IGF-binding protein 1; INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 1 PRECURSOR (IGFBP-1) (IBP-1) (IGF-BINDING PROTEIN 1)
• UniProt Protein Name: Insulin-like growth factor-binding protein 1
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: Igfbp1
• UniProt Synonym Gene Names: Igfbp-1; IBP-1; IGF-binding protein 1; IGFBP-1
• UniProt Entry Name: IBP1_MOUSE

Uniprot Description

IGFBP1: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; growth factor binding; insulin-like growth factor II binding

Biological Process: regulation of insulin-like growth factor receptor signaling pathway; regulation of cell growth