Typical Testing Data/Standard Curve (for reference only)
(Representative standard curve for IgE ELISA. IgE was diluted in serial two-fold steps in Sample Diluent.)
Mouse IgE ELISA Kit | IgE elisa kit
Mouse IgE ELISA KIT
Reactivity
Mouse
Synonyms
IgE; Mouse IgE ELISA KIT; Mouse IgE; IgE elisa kit
Reactivity
Mouse
Samples
Cell culture supernates, serum, and plasma
Assay Type
Quantitative Sandwich
Detection Range
312.5pglml- 20000 pg/ml
Sensitivity
156pglmL.
Preparation and Storage
Store at 4 degree C
Typical Testing Data/Standard Curve (for reference only)
(Representative standard curve for IgE ELISA. IgE was diluted in serial two-fold steps in Sample Diluent.)
Typical Testing Data/Standard Curve (for reference only)
(Representative standard curve for IgE ELISA. IgE was diluted in serial two-fold steps in Sample Diluent.)
Related Product Information for IgE elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme Immunoassay technique. A monoclonal antibody specific for IgE has been pre-coated onto a microplate. Standard, control or sample and the working solution of Biotin-Conjugate are p,petted into the wells. Following incubation and wash steps, any IgE present is bound by the immobilized antibody and the detection anlibody specific for IgE 'S binds to the combination of capture antibody- IgE In sample Following a wash to remove any unbound combination. and enzyme conjugate IS added to the wells Following incubation and wash steps a substrate IS added. A coloured product is formed in proporlion to the amount of IgE present In the sample The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve IS prepared from seven IgE standard dilutions and IgE sample concentration determined
Background/Introduction: There are five classes of mammalian immunoglobulins: IgA. IgD. IgE, IgG. and IgM. In mice. the IgG class is further divided into four subclasses' IgG1. IgG2a/lgG2c (strain specific). IgG2b. and IgG3.The general immunoglobulin structure is described as two heavy and two light chain (H2L2) polypeptides linked together by disu~ide bridges of cysteine residues. Mouse IgE. also known as reaginic antibody. exists in serum as a 4-chain monomer of 185-200 kDa. Like IgM. the IgE monomers contain a fourth constant region domain. Serum concentrations of IgE are reported to be >10 ug/ml. IgE is cyolytic for mast cells. where it binds to the Fc[1R 1 receptor and is able to mediate the response of granular and lipid mediators of inflammation. including the PCA reaction. The IgE class sWitch is induced by IL-4 as part of the Th2 response.
Background/Introduction: There are five classes of mammalian immunoglobulins: IgA. IgD. IgE, IgG. and IgM. In mice. the IgG class is further divided into four subclasses' IgG1. IgG2a/lgG2c (strain specific). IgG2b. and IgG3.The general immunoglobulin structure is described as two heavy and two light chain (H2L2) polypeptides linked together by disu~ide bridges of cysteine residues. Mouse IgE. also known as reaginic antibody. exists in serum as a 4-chain monomer of 185-200 kDa. Like IgM. the IgE monomers contain a fourth constant region domain. Serum concentrations of IgE are reported to be >10 ug/ml. IgE is cyolytic for mast cells. where it binds to the Fc[1R 1 receptor and is able to mediate the response of granular and lipid mediators of inflammation. including the PCA reaction. The IgE class sWitch is induced by IL-4 as part of the Th2 response.
Similar Products
Product Notes
The Mouse IgE (Catalog #AAA9424861) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9424861 ELISA Kit recognizes Mouse IgE. It is sometimes possible for the material contained within the vial of "IgE, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
