Human inhibitor of DNA binding 1, dominant negative helix-loop-helix protein ELISA Kit | ID1 elisa kit

Human DNA-binding protein inhibitor ID-1, ID1 ELISA Kit

Cat. Num. AAA911748

• Gene Names: ID1; ID; bHLHb24
• Reactivity: Human
• Synonyms: inhibitor of DNA binding 1; dominant negative helix-loop-helix protein; Human DNA-binding protein inhibitor ID-1; ID1 ELISA Kit; Human DNA-binding protein inhibitor ID-1 (ID1) ELISA kit; ID; bHLHb24; DNA-binding protein inhibitor ID-1; dJ857M17.1.2 (inhibitor of DNA binding 1; dominant negative helix-loop-helix protein) ; inhibitor of differentiation 1; ID1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human ID1. No significant cross-reactivity or interference between human ID1 and analogues was observed.
• Sequence Length: 155

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: 7.81 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ID1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ID1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ID1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ID1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ID1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 31317299
• NCBI GeneID: 3397
• NCBI Accession #: NP_002156.2
• NCBI GenBank Nucleotide #: NM_002165.3
• UniProt Accession #: P41134; O00651; O00652; Q16371; Q16377; Q5TE66; Q5TE67; Q969Z7; Q9H0Z5; Q9H109; A8K537; E1P5L4
• Molecular Weight: 16,133 Da
• NCBI Official Full Name: DNA-binding protein inhibitor ID-1 isoform a
• NCBI Official Synonym Full Names: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
• NCBI Official Symbol: ID1
• NCBI Official Synonym Symbols: ID; bHLHb24
• NCBI Protein Information: DNA-binding protein inhibitor ID-1; inhibitor of differentiation 1; class B basic helix-loop-helix protein 24; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein)
• UniProt Protein Name: DNA-binding protein inhibitor ID-1
• Protein Family: DNA-binding protein inhibitor
• UniProt Gene Name: ID1
• UniProt Synonym Gene Names: BHLHB24; ID; bHLHb24
• UniProt Entry Name: ID1_HUMAN

NCBI Description

The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ID1: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 20q11

Cellular Component: nucleoplasm; Golgi apparatus; nucleus

Molecular Function: protein dimerization activity; protein binding; transcription factor activity

Biological Process: circadian rhythm; transcription, DNA-dependent; collagen metabolic process; protein destabilization; blood vessel endothelial cell migration; heart development; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; regulation of angiogenesis; negative regulation of DNA binding; response to antibiotic; BMP signaling pathway; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; blood vessel morphogenesis; negative regulation of osteoblast differentiation; endothelial cell morphogenesis; angiogenesis; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis