Mouse Histone Deacetylase 4 (HDAC4) ELISA Kit | HDAC4 elisa kit

Mouse Histone Deacetylase 4 (HDAC4) ELISA Kit

Cat. Num. AAA2021580

• Gene Names: HDAC4; HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
• Reactivity: Mouse
• Synonyms: Histone Deacetylase 4 (HDAC4); Mouse Histone Deacetylase 4 (HDAC4) ELISA Kit; HD4; HDAC-A; HDACA; HDAC4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Histone Deacetylase 4 (HDAC4).; No significant cross-reactivity or interference between Histone Deacetylase 4 (HDAC4) and analogues was observed.
• Sequence Length: 972

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.129ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Deacetylase 4 (HDAC4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Deacetylase 4 (HDAC4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HDAC4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Deacetylase 4 (HDAC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Deacetylase 4 (HDAC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Deacetylase 4 (HDAC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone Deacetylase 4 (HDAC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for HDAC4 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 153085395
• NCBI GeneID: 9759
• NCBI Accession #: NP_006028.2
• NCBI GenBank Nucleotide #: NM_006037.3
• UniProt Accession #: P56524; Q86YH7; Q9UND6; E9PGB9; F5GX36
• Molecular Weight: 106,367 Da
• NCBI Official Full Name: histone deacetylase 4
• NCBI Official Synonym Full Names: histone deacetylase 4
• NCBI Official Symbol: HDAC4
• NCBI Official Synonym Symbols: HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
• NCBI Protein Information: histone deacetylase 4
• UniProt Protein Name: Histone deacetylase 4
• UniProt Gene Name: HDAC4
• UniProt Synonym Gene Names: KIAA0288; HD4
• UniProt Entry Name: HDAC4_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC4: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.

Protein type: Deacetylase; EC 3.5.1.98; Nuclear receptor co-regulator; Hydrolase

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: nucleoplasm; transcriptional repressor complex; histone deacetylase complex; cytoplasm; actomyosin; nucleus; Z disc; cytosol; neuromuscular junction; A band

Molecular Function: potassium ion binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); transcription activator binding; zinc ion binding; NAD-dependent histone deacetylase activity (H3-K14 specific); sequence-specific DNA binding; histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; protein kinase binding; transcription factor binding; transcription corepressor activity

Biological Process: response to drug; cardiac muscle hypertrophy; nervous system development; regulation of skeletal muscle fiber development; B cell activation; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; histone deacetylation; response to denervation involved in regulation of muscle adaptation; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; osteoblast development; positive regulation of protein sumoylation; chromatin remodeling; negative regulation of cell proliferation; regulation of protein binding; B cell differentiation; positive regulation of cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of osteoblast differentiation; inflammatory response; negative regulation of glycolysis; negative regulation of transcription, DNA-dependent; skeletal development