Mouse Hepcidin (Hepc) ELISA Kit | Hepc elisa kit

Mouse Hepcidin (Hepc) High Sensitive ELISA Kit

Cat. Num. AAA2708083

• Gene Names: Hamp; Hepc; Hamp1; Hepc1
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Hepcidin (Hepc); Mouse Hepcidin (Hepc) High Sensitive ELISA Kit; Hepcidin; HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor; Hepc elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Hepcidin (Hepc).; No significant cross-reactivity or interference between High Sensitive Hepcidin (Hepc) and analogues was observed.
• Sequence Length: 83

• Applicable Applications for Hepc elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 246.9-20000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 91.8pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Hepcidin (Hepc) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Hepcidin (Hepc) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Hepcidin (Hepc) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Hepcidin (Hepc) and unlabeled High Sensitive Hepcidin (Hepc) (Standards or samples) with the pre-coated antibody specific to High Sensitive Hepcidin (Hepc). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Hepcidin (Hepc) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Hepcidin (Hepc) in the sample.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 14211542
• NCBI GeneID: 84506
• NCBI Accession #: NP_115930.1
• NCBI GenBank Nucleotide #: NP_115930.1
• UniProt Accession #: Q9EQ21
• NCBI Official Full Name: hepcidin preproprotein
• NCBI Official Synonym Full Names: hepcidin antimicrobial peptide
• NCBI Official Symbol: Hamp
• NCBI Official Synonym Symbols: Hepc; Hamp1; Hepc1
• NCBI Protein Information: hepcidin
• UniProt Protein Name: Hepcidin
• Protein Family: Heparin-sulfate lyase
• UniProt Gene Name: Hamp
• UniProt Synonym Gene Names: Hamp1; Hepc; Hepc1

NCBI Description

This gene encodes hepcidin, an antimicrobial peptide and master hormonal regulator of systemic iron metabolism. The encoded preproprotein is synthesized in the hepatocytes where it undergoes proteolytic processing to generate disulfide-linked mature peptides that are secreted into the bloodstream. Mice lacking the encoded protein develop multivisceral iron overlaod, with sparing of the spleen macrophages. Certain mutations in the human ortholog of this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis. This gene is located adjacent to a related hepcidin gene on chromosome 7. [provided by RefSeq, Aug 2016]

Uniprot Description

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.