Western Blot (WB)
(Western Blot (WB) analysis of SRD5A1 polyclonal antibody at 1:500 dilutionLine1:HEK293T whole cell lysateLine2:H9C2 whole cell lysateLine3:Raw264. 7 whole cell lysate)
Rabbit SRD5A1 Polyclonal Antibody | anti-SRD5A1 antibody
SRD5A1 Polyclonal Antibody
Gene Names
SRD5A1; S5AR 1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SRD5A1; Polyclonal Antibody; SRD5A1 Polyclonal Antibody; 3-oxo-5-alpha-steroid 4-dehydrogenase 1 (EC: 1.3.1.22); SR type 1; Steroid 5-alpha-reductase 1; S5AR 1; anti-SRD5A1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SRD5A1 polyclonal antibody detects endogenous levels of SRD5A1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
259
Applicable Applications for anti-SRD5A1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human SRD5A1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of SRD5A1 polyclonal antibody at 1:500 dilutionLine1:HEK293T whole cell lysateLine2:H9C2 whole cell lysateLine3:Raw264. 7 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of SRD5A1 polyclonal antibody at 1:500 dilutionLine1:HEK293T whole cell lysateLine2:H9C2 whole cell lysateLine3:Raw264. 7 whole cell lysate)
Related Product Information for anti-SRD5A1 antibody
Steroid 5alpha-Reductase is an important enzyme in androgen physiology because it catalyzes the conversion of testosterone into the more potent 5alpha-dihydro-testosterone, which mediates androgen effects on target tissues. The enzyme exists as two isoforms: type 1, which is expressed mainly in the skin; and type 2, which is expressed mainly in the prostate. In cultured human skin cells, 5alpha-Reductase 1 shows heterogeneity of protein, and has different levels of transcriptional and translational expression. 5alpha-Reductase 1 is expressed in all portions of the hair follicle, whereas 5alpha-Reductase 2 is expressed only in mesenchymal portions. In addition, 5alpha-Reductase 1 is mainly expressed in human breast carcinoma and may play a role in the in situ production and actions of the potent androgen 5alpha-dihydrotestosterone, including inhibition of cancer cell proliferation in hormone-dependent human breast carcinoma. The 5alpha-Reductase-3alpha-hydroxysteroid dehydrogenase complex is present in the human brain, suggesting that the complex may be involved in the synthesis of neuroactive steroids or the catabolism of neurotoxic steroids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 29kDa
NCBI Official Full Name
3-oxo-5-alpha-steroid 4-dehydrogenase 1 isoform 1
NCBI Official Synonym Full Names
steroid 5 alpha-reductase 1
NCBI Official Symbol
SRD5A1
NCBI Official Synonym Symbols
S5AR 1
NCBI Protein Information
3-oxo-5-alpha-steroid 4-dehydrogenase 1
UniProt Protein Name
3-oxo-5-alpha-steroid 4-dehydrogenase 1
Protein Family
UniProt Gene Name
SRD5A1
UniProt Synonym Gene Names
S5AR 1
UniProt Entry Name
S5A1_HUMAN
NCBI Description
Steroid 5-alpha-reductase (EC 1.3.99.5) catalyzes the conversion of testosterone into the more potent androgen, dihydrotestosterone (DHT). Also see SRD5A2 (MIM 607306).[supplied by OMIM, Mar 2008]
Uniprot Description
SRD5A1: Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5- alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. Belongs to the steroid 5-alpha reductase family.
Protein type: Lipid Metabolism - androgen and estrogen; Membrane protein, integral; Oxidoreductase; Membrane protein, multi-pass; EC 1.3.1.22
Chromosomal Location of Human Ortholog: 5p15
Cellular Component: endoplasmic reticulum membrane; integral to membrane
Molecular Function: electron carrier activity; cholestenone 5-alpha-reductase activity; 3-oxo-5-alpha-steroid 4-dehydrogenase activity
Biological Process: steroid metabolic process; sex determination; androgen biosynthetic process; cell differentiation; sex differentiation
Research Articles on SRD5A1
1. Deficiency or inhibition of 5alphaR1 predisposes men and mice to insulin resistance and fatty liver.
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Product Notes
The SRD5A1 srd5a1 (Catalog #AAA3007331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SRD5A1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SRD5A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the SRD5A1 srd5a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRD5A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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