Typical Testing Data/Standard Curve (for reference only)
Mouse HDGF ELISA Kit | HDGF elisa kit
Mouse HDGF (Hepatoma Derived Growth Factor)ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HDGF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse HDGF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HDGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse HDGF. You can calculate the concentration of Mouse HDGF in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. This gene was thought initially to be located on chromosome X, however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
HDGF: Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor. Belongs to the HDGF family.
Protein type: Cytokine; Transcription regulation
Chromosomal Location of Human Ortholog: 1q23.1
Cellular Component: nucleoplasm; extracellular space; transcriptional repressor complex; cytoplasm
Molecular Function: heparin binding; DNA binding; growth factor activity; nucleotide binding
Biological Process: cell proliferation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; transcription, DNA-dependent; unfolded protein response; negative regulation of transcription from RNA polymerase II promoter; signal transduction
Research Articles on HDGF
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Product Notes
The Mouse HDGF hdgf (Catalog #AAA2504141) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2504141 ELISA Kit recognizes Mouse HDGF. It is sometimes possible for the material contained within the vial of "HDGF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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