Rat Hepatoma Derived Growth Factor ELISA Kit | HDGF elisa kit

Rat Hepatoma Derived Growth Factor ELISA Kit

Cat. Num. AAA725198

• Gene Names: HDGF; HMG1L2
• Reactivity: Rat
• Synonyms: Hepatoma Derived Growth Factor; Rat Hepatoma Derived Growth Factor ELISA Kit; HDGF elisa kit

• Reactivity: Rat

• Assay Type: Sandwich
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HDGF elisa kit

Intended Uses: This HDGF ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHDGF in the sample, thisHDGF ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHDGF concentration. The concentration ofHDGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This HDGF enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forHDGF. Standards or samples are then added to the microtiter plate wells andHDGF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofHDGF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forHDGF are added to each well to "sandwich" theHDGF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containHDGF and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Product Categories/Family for HDGF elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 4758516
• NCBI GeneID: 3068
• NCBI Accession #: NP_004485.1
• NCBI GenBank Nucleotide #: NM_004494.2
• Molecular Weight: 28,264 Da
• NCBI Official Full Name: hepatoma-derived growth factor isoform a
• NCBI Official Synonym Full Names: hepatoma-derived growth factor
• NCBI Official Symbol: HDGF
• NCBI Official Synonym Symbols: HMG1L2
• NCBI Protein Information: hepatoma-derived growth factor; HMG-1L2; high mobility group protein 1-like 2
• UniProt Protein Name: Hepatoma-derived growth factor
• Protein Family: Hepatoma-derived growth factor
• UniProt Gene Name: HDGF
• UniProt Synonym Gene Names: HMG1L2; HDGF; HMG-1L2
• UniProt Entry Name: HDGF_HUMAN

NCBI Description

This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. This gene was thought initially to be located on chromosome X, however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

HDGF: Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor. Belongs to the HDGF family.

Protein type: Transcription regulation; Cytokine

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: nucleoplasm; extracellular space; transcriptional repressor complex; cytoplasm

Molecular Function: heparin binding; DNA binding; growth factor activity; nucleotide binding

Biological Process: cell proliferation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; transcription, DNA-dependent; unfolded protein response; negative regulation of transcription from RNA polymerase II promoter; signal transduction