Mouse Glycosylated Lysosomal Membrane Protein (GLMP) ELISA Kit | GLMP elisa kit

Mouse Glycosylated Lysosomal Membrane Protein (GLMP) ELISA Kit

Cat. Num. AAA2707347

• Gene Names: GLMP; NCU-G1; C1orf85
• Reactivity: Mouse
• Synonyms: Glycosylated Lysosomal Membrane Protein (GLMP); Mouse Glycosylated Lysosomal Membrane Protein (GLMP) ELISA Kit; C1orf85; NCU-G1; Lysosomal protein NCU-G1; GLMP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glycosylated Lysosomal Membrane Protein (GLMP).; No significant cross-reactivity or interference between Glycosylated Lysosomal Membrane Protein (GLMP) and analogues was observed.
• Sequence Length: 325

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.123ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycosylated Lysosomal Membrane Protein (GLMP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycosylated Lysosomal Membrane Protein (GLMP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GLMP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycosylated Lysosomal Membrane Protein (GLMP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycosylated Lysosomal Membrane Protein (GLMP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycosylated Lysosomal Membrane Protein (GLMP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycosylated Lysosomal Membrane Protein (GLMP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 17511813
• NCBI GeneID: 112770
• UniProt Accession #: Q8WWB7; Q5SZX4; Q6UX96; Q8IV07; Q96F65; A6NH16; B4DJN4
• Molecular Weight: 34,991 Da
• NCBI Official Full Name: Chromosome 1 open reading frame 85
• NCBI Official Synonym Full Names: glycosylated lysosomal membrane protein
• NCBI Official Symbol: GLMP
• NCBI Official Synonym Symbols: NCU-G1; C1orf85
• NCBI Protein Information: glycosylated lysosomal membrane protein
• UniProt Protein Name: Glycosylated lysosomal membrane protein
• Protein Family: Glycosylated lysosomal membrane protein
• UniProt Gene Name: GLMP