Typical Testing Data/Standard Curve (for reference only)
Mouse Ghrelin (GHRL) ELISA Kit | GHRL elisa kit
Mouse Ghrelin (GHRL) Mini Samples ELISA Kit
Gene Names
Ghrl; Ghr; m46; MTLRP; MTLRPAP; 2210006E23Rik
Reactivity
Mouse
Applications
ELISA
Synonyms
Ghrelin (GHRL); Mouse Ghrelin (GHRL) Mini Samples ELISA Kit; Ghrelin; MTLRP; Growth Hormone-Releasing Peptide; GHRL elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Ghrelin (GHRL).
No significant cross-reactivity or interference between Mini Samples Ghrelin (GHRL) and analogues was observed.
No significant cross-reactivity or interference between Mini Samples Ghrelin (GHRL) and analogues was observed.
Sequence Length
117
Applicable Applications for GHRL elisa kit
ELISA (EIA)
Assay Type
Double-antibody Sandwich
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
123.5-10,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 49.2pg/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Ghrelin (GHRL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Ghrelin (GHRL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Ghrelin (GHRL) and unlabeled Mini Samples Ghrelin (GHRL) (Standards or samples) with the pre-coated antibody specific to Mini Samples Ghrelin (GHRL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Ghrelin (GHRL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Ghrelin (GHRL) in the sample.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
appetite-regulating hormone isoform 1 preproprotein
NCBI Official Synonym Full Names
ghrelin
NCBI Official Symbol
Ghrl
NCBI Official Synonym Symbols
Ghr; m46; MTLRP; MTLRPAP; 2210006E23Rik
NCBI Protein Information
appetite-regulating hormone
UniProt Protein Name
Appetite-regulating hormone
Protein Family
UniProt Gene Name
Ghrl
UniProt Synonym Gene Names
Mtlrp
UniProt Entry Name
GHRL_MOUSE
NCBI Description
This gene encodes a preproprotein that undergoes proteolytic processing to yield two bioactive peptides, ghrelin and obestatin. The hormone ghrelin plays a role in enhancing appetite and has numerous other biological functions that include stimulating the secretion of growth hormone (somatotropin) from the anterior pituitary gland. Obestatin is thought to be a hormone that functions in decreasing appetite. Mice lacking the encoded protein develop normally and exhibit no gross anatomical abnormalities. This gene encodes distinct isoforms, some or all of which may undergo similar proteolytic processing. [provided by RefSeq, Jul 2016]
Research Articles on GHRL
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Product Notes
The Mouse GHRL ghrl (Catalog #AAA2708338) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2708338 ELISA Kit recognizes Mouse GHRL. AAA Biotech's Ghrelin (GHRL) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the GHRL ghrl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ghrelin (GHRL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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