Mouse Ghrelin (GHRL) RTU ELISA Kit | GHRL rtu elisa kit

Mouse Ghrelin (GHRL) ELISA Kit

Cat. Num. AAA459578

• Gene Names: Ghrl; Ghr; m46; MTLRP; MTLRPAP; 2210006E23Rik
• Reactivity: Mouse
• Synonyms: Ghrelin (GHRL); Mouse Ghrelin (GHRL) ELISA Kit; MTLRP; Growth Hormone-Releasing Peptide; GHRL rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 116

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.042ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GHRL rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GHRL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GHRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GHRL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 557357757
• NCBI GeneID: 58991
• NCBI Accession #: NP_001273333.1
• NCBI GenBank Nucleotide #: NM_001286404.1
• UniProt Accession #: Q9EQX0; Q9WUZ1
• Molecular Weight: 13,079 Da
• NCBI Official Full Name: appetite-regulating hormone isoform 2 preproprotein
• NCBI Official Synonym Full Names: ghrelin
• NCBI Official Symbol: Ghrl
• NCBI Official Synonym Symbols: Ghr; m46; MTLRP; MTLRPAP; 2210006E23Rik
• NCBI Protein Information: appetite-regulating hormone
• UniProt Protein Name: Appetite-regulating hormone
• Protein Family: Ghrelin
• UniProt Gene Name: Ghrl
• UniProt Synonym Gene Names: Mtlrp

NCBI Description

This gene encodes a preproprotein that undergoes proteolytic processing to yield two bioactive peptides, ghrelin and obestatin. The hormone ghrelin plays a role in enhancing appetite and has numerous other biological functions that include stimulating the secretion of growth hormone (somatotropin) from the anterior pituitary gland. Obestatin is thought to be a hormone that functions in decreasing appetite. Mice lacking the encoded protein develop normally and exhibit no gross anatomical abnormalities. This gene encodes distinct isoforms, some or all of which may undergo similar proteolytic processing. [provided by RefSeq, Jul 2016]

Uniprot Description

Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility ().