Mouse GDNF family receptor alpha-4 (GFRA4) ELISA Kit | GFRA4 elisa kit

Mouse GDNF family receptor alpha-4 (GFRA4) ELISA Kit

Cat. Num. AAA287638

• Gene Names: Gfra4; AW060733; G630015H18Rik
• Reactivity: Mouse
• Synonyms: GDNF family receptor alpha-4 (GFRA4); Mouse GDNF family receptor alpha-4 (GFRA4) ELISA Kit; RP5-964F7.1; GFR receptor alpha 4; persephin receptor; GFRA4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse GFRA4. No significant cross-reactivity or interference between Mouse GFRA4 and analogues was observed.
• Sequence Length: 293

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for GFRA4 elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate GFRA4 in samples. An antibody specific for GFRA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GFRA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GFRA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GFRA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: GFRa4 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for persephin, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for RET-associated diseases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. GFRalpha-4 is substantially different in sequence from all known GFRalphas, including chicken GFRalpha-4, and lacks the first cysteine-rich domain present in all previously characterized GFRalphas. At least two different GFRalpha-4 splice variants exist in rat tissues, differing at their respective COOH termini. GFRalpha-4 mRNA is expressed at low levels in different brain areas in the adult as well as in some peripheral tissues including testis and heart.

NCBI and Uniprot Product Information

• NCBI GI #: 209862933
• NCBI GeneID: 14588
• NCBI Accession #: NP_001129535.1
• NCBI GenBank Nucleotide #: NM_001136063.2
• UniProt Accession #: Q9JJT2; Q9JJT3; Q9JJT4; Q9JJT6; Q9JJT7; Q9JJT8; A2AP44; A2AP46; A2AP47; A2AP49
• Molecular Weight: 21,266 Da
• NCBI Official Full Name: GDNF family receptor alpha-4 isoform 1
• NCBI Official Synonym Full Names: glial cell line derived neurotrophic factor family receptor alpha 4
• NCBI Official Symbol: Gfra4
• NCBI Official Synonym Symbols: AW060733; G630015H18Rik
• NCBI Protein Information: GDNF family receptor alpha-4
• UniProt Protein Name: GDNF family receptor alpha-4
• Protein Family: GDNF family receptor
• UniProt Gene Name: Gfra4
• UniProt Synonym Gene Names: GDNF receptor alpha-4; GDNFR-alpha-4; GFR-alpha-4

NCBI Description

This gene encodes a transmembrane protein that functions as the receptor for persephin, a member of the glial cell line derived neurotrophic factors. The encoded protein undergoes proteolytic processing to generate a glycosylphosphatidylinositol-anchored cell surface coreceptor that forms a complex with the receptor tyrosine kinase Ret. A complete lack of the encoded protein impairs production of thyroid calcitonin and increases the rate of bone formation in young mice. [provided by RefSeq, Jul 2016]

Uniprot Description

Receptor for persephin. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor. May be important in C-cell development and, in the postnatal development of the adrenal medulla.