Mouse G Protein Coupled Receptor Kinase 5 (GRK5) ELISA Kit | GRK5 elisa kit

Mouse G Protein Coupled Receptor Kinase 5 (GRK5) ELISA Kit

Cat. Num. AAA2705006

• Gene Names: GRK5; GPRK5
• Reactivity: Mouse
• Synonyms: G Protein Coupled Receptor Kinase 5 (GRK5); Mouse G Protein Coupled Receptor Kinase 5 (GRK5) ELISA Kit; GPRK5; G protein-coupled receptor kinase GRK5; GRK5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of G Protein Coupled Receptor Kinase 5 (GRK5).; No significant cross-reactivity or interference between G Protein Coupled Receptor Kinase 5 (GRK5) and analogues was observed.
• Sequence Length: 590

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level G Protein Coupled Receptor Kinase 5 (GRK5) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level G Protein Coupled Receptor Kinase 5 (GRK5) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRK5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Receptor Kinase 5 (GRK5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Receptor Kinase 5 (GRK5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Receptor Kinase 5 (GRK5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Coupled Receptor Kinase 5 (GRK5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4885349
• NCBI GeneID: 2869
• NCBI Accession #: NP_005299.1
• NCBI GenBank Nucleotide #: NM_005308.2
• UniProt Accession #: P34947; Q5T059; D3DRD0
• Molecular Weight: 67,787 Da
• NCBI Official Full Name: G protein-coupled receptor kinase 5
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 5
• NCBI Official Symbol: GRK5
• NCBI Official Synonym Symbols: GPRK5
• NCBI Protein Information: G protein-coupled receptor kinase 5
• UniProt Protein Name: G protein-coupled receptor kinase 5
• Protein Family: G protein-coupled receptor kinase
• UniProt Gene Name: GRK5
• UniProt Synonym Gene Names: GPRK5

NCBI Description

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008]

Uniprot Description

GRK5: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein- coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70- interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2- mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G- protein-coupled receptor, LRP6 during Wnt signaling (in vitro). Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Protein type: AGC group; EC 2.7.11.16; GRK family; GRK subfamily; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: cytoplasm; cytosol; nuclear membrane; nuclear speck; plasma membrane

Molecular Function: ATP binding; beta-adrenergic receptor kinase activity; G-protein coupled receptor kinase activity; phospholipid binding; protein binding; protein kinase C binding; protein serine/threonine kinase activity

Biological Process: apoptosis; fat cell differentiation; G-protein coupled receptor signaling pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; negative regulation of apoptosis; positive regulation of cell proliferation; protein autophosphorylation; regulation of cell cycle; regulation of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway; Wnt signaling pathway