Mouse FVII ELISA Kit | FVII elisa kit

Mouse FVII(Coagulation Factor VII)ELISA Kit 

Cat. Num. AAA2515890

• Gene Names: F7; SPCA
• Reactivity: Mouse
• Synonyms: FVII; Mouse FVII(Coagulation Factor VII)ELISA Kit ; FVII elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinantMouseFVII. No significant cross-reactivity or interference between MouseFVII and analogues was observed.
• Sequence Length: 466

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FVII elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseFVIIconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FVII. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FVIIand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FVII, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FVII.You can calculate the concentration of FVIIin the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 37781363
• NCBI GeneID: 2155
• Molecular Weight: 49,320 Da
• NCBI Official Full Name: FVII coagulation protein
• NCBI Official Synonym Full Names: coagulation factor VII (serum prothrombin conversion accelerator)
• NCBI Official Symbol: F7
• NCBI Official Synonym Symbols: SPCA
• NCBI Protein Information: coagulation factor VII; FVII coagulation protein; eptacog alfa; proconvertin
• UniProt Protein Name: Coagulation factor VII
• UniProt Gene Name: F7
• UniProt Synonym Gene Names: SPCA
• UniProt Entry Name: FA7_HUMAN

NCBI Description

This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

F7: Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium. Defects in F7 are the cause of factor VII deficiency (FA7D). A hemorrhagic disease with variable presentation. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or repeated hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Finally, numerous subjects are completely asymptomatic despite very low factor VII levels. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Secreted, signal peptide; Secreted; Apoptosis; Motility/polarity/chemotaxis; EC 3.4.21.21

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: extracellular space; endoplasmic reticulum lumen; Golgi lumen; plasma membrane; extracellular region; vesicle

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity; calcium ion binding; glycoprotein binding; receptor binding

Biological Process: circadian rhythm; organ regeneration; positive regulation of positive chemotaxis; positive regulation of blood coagulation; positive regulation of leukocyte chemotaxis; proteolysis; post-translational protein modification; peptidyl-glutamic acid carboxylation; positive regulation of protein kinase B signaling cascade; blood coagulation, extrinsic pathway; cellular protein metabolic process; response to vitamin K; response to estrogen stimulus; blood coagulation; positive regulation of cell migration

Disease: Myocardial Infarction, Susceptibility To; Factor Vii Deficiency