Mouse Follicle Stimulating Hormone (FSH) ELISA Kit | FSH elisa kit
Mouse Follicle Stimulating Hormone (FSH) ELISA Kit
Gene Names
Fshb; FSH; FSH-B; Fshbeta; FSH-beta
Reactivity
Mouse
Synonyms
Follicle Stimulating Hormone (FSH); Mouse Follicle Stimulating Hormone (FSH) ELISA Kit; FSH elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of FSH. No significant cross-reactivity or interference between FSH and analogues was observed.
Sequence Length
130
Samples
Serum, plasma and other biological fluids.
Assay Type
Competitive inhibition
Detection Range
3.125-200ng/mL
Sensitivity
1.49ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FSH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FSH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Related Product Information for FSH elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of FSH in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to FSH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled FSH and unlabeled FSH (Standards or samples) with the pre-coated antibody specific to FSH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of FSH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of FSH in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to FSH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled FSH and unlabeled FSH (Standards or samples) with the pre-coated antibody specific to FSH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of FSH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of FSH in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,919 Da
NCBI Official Full Name
follitropin subunit beta
NCBI Official Synonym Full Names
follicle stimulating hormone beta
NCBI Official Symbol
Fshb
NCBI Official Synonym Symbols
FSH; FSH-B; Fshbeta; FSH-beta
NCBI Protein Information
follitropin subunit beta
UniProt Protein Name
Follitropin subunit beta
UniProt Gene Name
Fshb
UniProt Synonym Gene Names
FSH-B; FSH-beta
NCBI Description
The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. [provided by RefSeq, Aug 2015]
Uniprot Description
Stimulates development of follicle and spermatogenesis in the reproductive organs.
Research Articles on FSH
Similar Products
Product Notes
The Mouse FSH fshb (Catalog #AAA452569) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA452569 ELISA Kit recognizes Mouse FSH. It is sometimes possible for the material contained within the vial of "Follicle Stimulating Hormone (FSH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
