Mouse FLT1/VEGFR1 ELISA Kit | Flt1 elisa kit

Mouse FLT1/VEGFR1 PicoKine ELISA Kit

Cat. Num. AAA178718

• Gene Names: Flt1; Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757
• Reactivity: Mouse
• Synonyms: FLT1/VEGFR1; Mouse FLT1/VEGFR1 PicoKine ELISA Kit; FLT 1; FLT1; Flt-1; sflt 1; sFLT1; Soluble VEGFR1 variant 2; Soluble VEGFR1 variant 21; sVEGFR1; Tyrosine protein kinase FRT; Tyrosine-protein kinase FRT; VEGF Receptor 1; VEGFR 1; VEGFR-1; VGFR1; Vascular endothelial growth factor receptor 1; FMS-like tyrosine kinase 1; Flt1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Flt1
• Sequence Length: 1333

• Samples: Cell culture supernatants and serum
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Flt1 elisa kit

Principle of the Assay: The Picokine™ Mouse Flt1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Flt1 with a 96-well strip plate that is pre-coated with antibody specific for Flt1. The detection antibody is a biotinylated antibody specific for Flt1. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Flt1 with immunogen: Expression system for standard: NSO, Immunogen sequence: Y23-E759. The kit is analytically validated with ready to use reagents. To measure Mouse Flt1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Flt1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Flt1 in the sample.

Background/Introduction: Vascular endothelial growth factor receptor 1 is a protein that in humans is encoded by the FLT1 gene. Oncogene FLT belongs to the src gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.

NCBI and Uniprot Product Information

• NCBI GI #: 34328180
• NCBI GeneID: 14254
• NCBI Accession #: NP_034358.2
• NCBI GenBank Nucleotide #: NM_010228.3
• UniProt Accession #: P35969; O55094; Q61517
• Molecular Weight: 149,876 Da
• NCBI Official Full Name: vascular endothelial growth factor receptor 1
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 1
• NCBI Official Symbol: Flt1
• NCBI Official Synonym Symbols: Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757
• NCBI Protein Information: vascular endothelial growth factor receptor 1
• UniProt Protein Name: Vascular endothelial growth factor receptor 1
• Protein Family: Vascular endothelial growth factor receptor
• UniProt Gene Name: Flt1
• UniProt Synonym Gene Names: Emrk2; Flt; Vegfr1; VEGFR-1; FLT-1

Uniprot Description

VEGFR1: a receptor tyrosine kinase of the VEGFR family. Receptor for VEGF, VEGFB and PGF. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Two splice variant isoforms have been described. Isoform SFlt1 may have an inhibitory role in angiogenesis.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; VEGFR family

Chromosomal Location of Human Ortholog: 5 G3|5 87.01 cM

Cellular Component: extracellular space; focal adhesion; integral to plasma membrane; nucleus; receptor complex

Molecular Function: growth factor binding; identical protein binding; vascular endothelial growth factor receptor activity

Biological Process: angiogenesis; blood vessel morphogenesis; cell migration; embryonic morphogenesis; monocyte chemotaxis; patterning of blood vessels; peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; positive regulation of cell migration; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of vascular endothelial growth factor receptor signaling pathway; post-embryonic camera-type eye morphogenesis; protein amino acid autophosphorylation; response to hypoxia; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway