Mouse Filamin Binding LIM Protein 1 (FBLIM1) ELISA Kit | FBLIM1 elisa kit

Mouse Filamin Binding LIM Protein 1 (FBLIM1) ELISA Kit

Cat. Num. AAA457032

• Gene Names: Fblim1; Cal; Gt10; Fblp1; migfilin; migfilin(s); 2410043F08Rik
• Reactivity: Mouse
• Synonyms: Filamin Binding LIM Protein 1 (FBLIM1); Mouse Filamin Binding LIM Protein 1 (FBLIM1) ELISA Kit; CAL; FBLP-1; FBLP1; Migfilin; Mitogen-inducible 2-interacting protein; FBLIM1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of FBLIM1. No significant cross-reactivity or interference between FBLIM1 and analogues was observed.
• Sequence Length: 375

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.053ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FBLIM1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FBLIM1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBLIM1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of FBLIM1 in mouse serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FBLIM1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FBLIM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FBLIM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FBLIM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 253795477
• NCBI GeneID: 74202
• NCBI Accession #: NP_001156728.1
• NCBI GenBank Nucleotide #: NM_001163256.1
• UniProt Accession #: Q71FD7; Q3TDK3; Q99J35
• Molecular Weight: 41,026 Da
• NCBI Official Full Name: filamin-binding LIM protein 1
• NCBI Official Synonym Full Names: filamin binding LIM protein 1
• NCBI Official Symbol: Fblim1
• NCBI Official Synonym Symbols: Cal; Gt10; Fblp1; migfilin; migfilin(s); 2410043F08Rik
• NCBI Protein Information: filamin-binding LIM protein 1
• UniProt Protein Name: Filamin-binding LIM protein 1
• Protein Family: Filamin-binding LIM protein
• UniProt Gene Name: Fblim1
• UniProt Synonym Gene Names: Cal; FBLP-1

Uniprot Description

Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin-containing signaling complexes that control actin assembly. Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion ().