Mouse Fibroblast Growth Factor 5 (FGF5) ELISA Kit | FGF5 elisa kit

Mouse Fibroblast Growth Factor 5 (FGF5) Mini Samples ELISA Kit

Cat. Num. AAA2708290

• Gene Names: Fgf5; go; Fgf-5; HBGF-5; angora
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Fibroblast Growth Factor 5 (FGF5); Mouse Fibroblast Growth Factor 5 (FGF5) Mini Samples ELISA Kit; Fibroblast Growth Factor 5; HBGF5; Smag82; Heparin-binding growth factor 5; FGF5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Fibroblast Growth Factor 5 (FGF5).; No significant cross-reactivity or interference between Mini Samples Fibroblast Growth Factor 5 (FGF5) and analogues was observed.
• Sequence Length: 121

• Applicable Applications for FGF5 elisa kit: ELISA (EIA)
• Assay Type: Competitive Inhibition
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 7.8-500pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 2.9pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Fibroblast Growth Factor 5 (FGF5) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Fibroblast Growth Factor 5 (FGF5) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Fibroblast Growth Factor 5 (FGF5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Fibroblast Growth Factor 5 (FGF5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Fibroblast Growth Factor 5 (FGF5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Fibroblast Growth Factor 5 (FGF5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 471270260
• NCBI GeneID: 14176
• NCBI Accession #: NP_001264197.1
• NCBI GenBank Nucleotide #: NP_001264197.1
• UniProt Accession #: P15656
• NCBI Official Full Name: fibroblast growth factor 5 isoform 2
• NCBI Official Synonym Full Names: fibroblast growth factor 5
• NCBI Official Symbol: Fgf5
• NCBI Official Synonym Symbols: go; Fgf-5; HBGF-5; angora
• NCBI Protein Information: fibroblast growth factor 5
• UniProt Protein Name: Fibroblast growth factor 5
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: Fgf5
• UniProt Synonym Gene Names: Fgf-5; FGF-5; HBGF-5
• UniProt Entry Name: FGF5_MOUSE

NCBI Description

This gene encodes a secreted protein that is a member of a family of heparin-binding growth factors. The encoded protein regulates cell proliferation, particularly the growth of hair follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Uniprot Description

FGF5: Plays an important role in the regulation of cell proliferation and cell differentiation. Required for normal regulation of the hair growth cycle. Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Oncoprotein; Cytokine

Cellular Component: extracellular region

Molecular Function: growth factor activity; receptor binding; fibroblast growth factor receptor binding

Biological Process: cell proliferation; fibroblast growth factor receptor signaling pathway; glial cell differentiation; positive regulation of cell division; positive regulation of cell proliferation