Mouse F-Box And Leucine Rich Repeat Protein 3 (FBXL3) ELISA Kit | FBXL3 elisa kit

Mouse F-Box And Leucine Rich Repeat Protein 3 (FBXL3) ELISA Kit

Cat. Num. AAA2706591

• Gene Names: FBXL3; FBL3; FBL3A; FBXL3A
• Reactivity: Mouse
• Synonyms: F-Box And Leucine Rich Repeat Protein 3 (FBXL3); Mouse F-Box And Leucine Rich Repeat Protein 3 (FBXL3) ELISA Kit; FBL3; FBL3A; FBXL3A; F-box/LRR-repeat protein 3A; FBXL3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of F-Box And Leucine Rich Repeat Protein 3 (FBXL3).; No significant cross-reactivity or interference between F-Box And Leucine Rich Repeat Protein 3 (FBXL3) and analogues was observed.
• Sequence Length: 428

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level F-Box And Leucine Rich Repeat Protein 3 (FBXL3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level F-Box And Leucine Rich Repeat Protein 3 (FBXL3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBXL3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to F-Box And Leucine Rich Repeat Protein 3 (FBXL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to F-Box And Leucine Rich Repeat Protein 3 (FBXL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F-Box And Leucine Rich Repeat Protein 3 (FBXL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F-Box And Leucine Rich Repeat Protein 3 (FBXL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119600969
• NCBI GeneID: 26224
• UniProt Accession #: Q9UKT7; Q9P122; B2RB04
• Molecular Weight: 48,707 Da
• NCBI Official Full Name: F-box and leucine-rich repeat protein 3
• NCBI Official Synonym Full Names: F-box and leucine rich repeat protein 3
• NCBI Official Symbol: FBXL3
• NCBI Official Synonym Symbols: FBL3; FBL3A; FBXL3A
• NCBI Protein Information: F-box/LRR-repeat protein 3
• UniProt Protein Name: F-box/LRR-repeat protein 3
• UniProt Gene Name: FBXL3
• UniProt Synonym Gene Names: FBL3A; FBXL3A

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats and is localized in the nucleus. [provided by RefSeq, Jul 2008]

Uniprot Description

Substrate-recognition component of the SCF(FBXL3) E3 ubiquitin ligase complex involved in circadian rhythm function. Plays a key role in the maintenance of both the speed and the robustness of the circadian clock oscillation (PubMed:17463251, PubMed:23452855, PubMed:27565346). The SCF(FBXL3) complex mainly acts in the nucleus and mediates ubiquitination and subsequent degradation of CRY1 and CRY2 (PubMed:17463251, PubMed:23452855, PubMed:27565346). Activity of the SCF(FBXL3) complex is counteracted by the SCF(FBXL21) complex (PubMed:23452855).