Mouse Exostosin-2 (EXT2) ELISA Kit | EXT2 elisa kit
Mouse Exostosin-2 (EXT2) ELISA Kit
Gene Names
Ext2; AI893565
Reactivity
Mouse
Synonyms
Exostosin-2 (EXT2); Mouse Exostosin-2 (EXT2) ELISA Kit; SOTV; Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N- acetylglucosaminyltransferase; N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase; exostosin 2; EXT2 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse EXT2. No significant cross-reactivity or interference between Mouse EXT2 and analogues was observed.
Sequence Length
718
Assay Type
Quantitive Sandwich
Samples
Serum, Plasma, Other Biological Fluids
Detection Wavelength
450 nm
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%)
= SD/meanX100
Intra-Assay
CV
Inter-Assay
CV
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for EXT2 elisa kit
Description: This assay employs a two-site sandwich ELISA to quantitate EXT2 in samples. An antibody specific for EXT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EXT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EXT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EXT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Exostosin-2 is one of two glycosyltransferases involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type II form of multiple exostoses.By positional cloning and use of a chromosome 11-specific fetal brain cDNA library, Stickens et al. (1996) identified the putative EXT2 gene, which was predicted to encode a 718-amino acid protein with a molecular mass of 82 kD. Both protein and DNA sequence-based algorithms detected significant similarity with the product of the EXT1 gene, suggesting that the 2 genes are evolutionarily and functionally related. Northern blot analysis detected a major 3.5-kb mRNA transcript and a minor band of 3.7 to 4.0 kb in most tissues. EXT2 gene may be expressed as a complex set of alternatively spliced forms.
Overview: Exostosin-2 is one of two glycosyltransferases involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type II form of multiple exostoses.By positional cloning and use of a chromosome 11-specific fetal brain cDNA library, Stickens et al. (1996) identified the putative EXT2 gene, which was predicted to encode a 718-amino acid protein with a molecular mass of 82 kD. Both protein and DNA sequence-based algorithms detected significant similarity with the product of the EXT1 gene, suggesting that the 2 genes are evolutionarily and functionally related. Northern blot analysis detected a major 3.5-kb mRNA transcript and a minor band of 3.7 to 4.0 kb in most tissues. EXT2 gene may be expressed as a complex set of alternatively spliced forms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
82,064 Da
NCBI Official Full Name
exostosin-2 isoform 2
NCBI Official Synonym Full Names
exostoses (multiple) 2
NCBI Official Symbol
Ext2
NCBI Official Synonym Symbols
AI893565
NCBI Protein Information
exostosin-2
UniProt Protein Name
Exostosin-2
Protein Family
UniProt Gene Name
Ext2
Uniprot Description
Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor. Required for the exosomal release of SDCBP, CD63 and syndecan.
Research Articles on EXT2
Similar Products
Product Notes
The Mouse EXT2 ext2 (Catalog #AAA2800139) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2800139 ELISA Kit recognizes Mouse EXT2. It is sometimes possible for the material contained within the vial of "Exostosin-2 (EXT2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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