Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Epoxide hydrolase 4 (EPHX4) Standard Curve.)
Mouse Epoxide Hydrolase 4 (EPHX4) ELISA Kit | EPHX4 elisa kit
Mouse Epoxide Hydrolase 4 (EPHX4) ELISA Kit
Gene Names
Ephx4; Abhd7; Ephxrp; Gm1382
Reactivity
Mouse
Synonyms
Epoxide Hydrolase 4 (EPHX4); Mouse Epoxide Hydrolase 4 (EPHX4) ELISA Kit; EPHX4; ABHD7; EPHXRP; FLJ90341; abhydrolase domain containing 7; EPHX4 elisa kit
Reactivity
Mouse
Specificity
Mouse Epoxide hydrolase 4 (EPHX4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse EPHX4. No significant cross-reactivity or interference between Mouse EPHX4 and analogues was observed.
Sequence Length
359
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Epoxide hydrolase 4 (EPHX4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Epoxide hydrolase 4 (EPHX4) Standard Curve.)
Related Product Information for EPHX4 elisa kit
Description: This Mouse Epoxide hydrolase 4 (EPHX4) ELISA Kit employs a two-site sandwich ELISA to quantitate EPHX4 in samples. An antibody specific for EPHX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPHX4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPHX4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPHX4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: EPHX4 belongs to the AB hydrolase superfamily.In contrast to other members of the AB hydrolase superfamily, lacks the conserved Ser residue at position 169, which is replaced by an Asp residue, suggesting it has no hydrolase activity. Epoxide hydrolase (also known as epoxide hydratase) functions in detoxication during drug metabolism. It converts epoxides to trans-dihydrodiols, which can be conjugated and excreted from the body. Epoxides result from the degradation of aromatic compounds. Epoxides are significant as cytochrome P450 oxidase metabolites of unsaturated carbon-carbon bonds, but are also mutagenic. Epoxide hydrolase is present in large quantity on endoplasmic reticulum.
Background: EPHX4 belongs to the AB hydrolase superfamily.In contrast to other members of the AB hydrolase superfamily, lacks the conserved Ser residue at position 169, which is replaced by an Asp residue, suggesting it has no hydrolase activity. Epoxide hydrolase (also known as epoxide hydratase) functions in detoxication during drug metabolism. It converts epoxides to trans-dihydrodiols, which can be conjugated and excreted from the body. Epoxides result from the degradation of aromatic compounds. Epoxides are significant as cytochrome P450 oxidase metabolites of unsaturated carbon-carbon bonds, but are also mutagenic. Epoxide hydrolase is present in large quantity on endoplasmic reticulum.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
epoxide hydrolase 4
NCBI Official Synonym Full Names
epoxide hydrolase 4
NCBI Official Symbol
Ephx4
NCBI Official Synonym Symbols
Abhd7; Ephxrp; Gm1382
NCBI Protein Information
epoxide hydrolase 4
UniProt Protein Name
Epoxide hydrolase 4
Protein Family
UniProt Gene Name
Ephx4
UniProt Synonym Gene Names
Abhd7; Ephxrp
Similar Products
Product Notes
The Mouse EPHX4 ephx4 (Catalog #AAA9717998) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717998 ELISA Kit recognizes Mouse EPHX4. It is sometimes possible for the material contained within the vial of "Epoxide Hydrolase 4 (EPHX4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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