Related Product Information for Eotaxin 1 elisa kit
Principle of the Assay: EOTAXIN 1 ELIS A kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for EOTAXIN 1. Standards or samples are then added to the microtiter plate wells and EOTAXIN 1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of EOTAXIN 1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for EOTAXIN 1 are added to each well to "sandwich" the EOTAXIN 1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain EOTAXIN 1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotomehically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EOTAXIN 1 concentration in each sample is interpolated from this standard curve.
NCBI Description
This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil-specific chemokine is thought to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. [provided by RefSeq, Sep 2014]
Uniprot Description
CCL11: In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: extracellular space; extracellular region
Molecular Function: protein binding; chemokine activity
Biological Process: chronic inflammatory response; mast cell chemotaxis; response to virus; actin filament organization; cytoskeleton organization and biogenesis; signal transduction; chemotaxis; protein amino acid phosphorylation; cellular calcium ion homeostasis; regulation of cell shape; response to radiation; positive regulation of angiogenesis; positive regulation of actin filament polymerization; eosinophil chemotaxis; positive regulation of endothelial cell proliferation; immune response; inflammatory response; cell adhesion; positive regulation of cell migration
Disease: Asthma, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To
Product Notes
The Mouse Eotaxin 1 ccl11 (Catalog #
AAA736922) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA736922 ELISA Kit recognizes Mouse Eotaxin 1.
It is sometimes possible for the material contained within the vial of
"Eotaxin 1, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are
absolutely not suitable for use in any
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industry standard. AAA Biotech
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