Mouse Eosinophil Peroxidase (EPX) ELISA Kit | EPX elisa kit

Mouse Eosinophil Peroxidase (EPX) ELISA Kit

Cat. Num. AAA2706597

• Gene Names: EPX; EPO; EPP; EPXD; EPX-PEN
• Reactivity: Mouse
• Synonyms: Eosinophil Peroxidase (EPX); Mouse Eosinophil Peroxidase (EPX) ELISA Kit; EPO; EPP; EPX-PEN; EPER; EPX elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of EPX. No significant cross-reactivity or interference between EPX and analogues was observed.
• Sequence Length: 715

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: < 6.0pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EPX were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EPX were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for EPX elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EPX in mouse serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to EPX. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to EPX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EPX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EPX in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 182146
• NCBI GeneID: 8288
• UniProt Accession #: P11678; Q4TVP3
• Molecular Weight: 81,040 Da
• NCBI Official Full Name: eosinophil peroxidase
• NCBI Official Synonym Full Names: eosinophil peroxidase
• NCBI Official Symbol: EPX
• NCBI Official Synonym Symbols: EPO; EPP; EPXD; EPX-PEN
• NCBI Protein Information: eosinophil peroxidase
• UniProt Protein Name: Eosinophil peroxidase
• Protein Family: Eosinophil peroxidase
• UniProt Gene Name: EPX
• UniProt Synonym Gene Names: EPER; EPO; EPP; EPO

NCBI Description

This gene is a member of the peroxidase gene family and is expressed in eosinophils. The encoded preproprotein is proteolytically processed into covalently attached heavy and light chains to form the mature enzyme, which functions as an oxidant. The enzyme is released at sites of parasitic infection or allergen stimulation to mediate lysis of protozoa or parasitic worms. The gene is found in a gene cluster with other peroxidase genes on chromosome 17. Mutations in this gene result in eosinophil peroxidase deficiency. [provided by RefSeq, Feb 2016]

Uniprot Description

Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis.