Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse E3 ubiquitin-protein ligase TRIM68 (TRIM68) Standard Curve.)
Mouse E3 Ubiquitin-Protein Ligase TRIM68 (TRIM68) ELISA Kit | TRIM68 elisa kit
Mouse E3 Ubiquitin-Protein Ligase TRIM68 (TRIM68) ELISA Kit
Reactivity
Mouse
Synonyms
E3 Ubiquitin-Protein Ligase TRIM68 (TRIM68); Mouse E3 Ubiquitin-Protein Ligase TRIM68 (TRIM68) ELISA Kit; TRIM68; FLJ10369; GC109; MGC126176; RNF137; SS-56; SS56; Ro/SSA1 related protein; SSA protein SS-56; ring finger protein 137; TRIM68 elisa kit
Reactivity
Mouse
Specificity
Mouse E3 ubiquitin-protein ligase TRIM68 (TRIM68) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TRIM68. No significant cross-reactivity or interference between Mouse TRIM68 and analogues was observed.
Sequence Length
485
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse E3 ubiquitin-protein ligase TRIM68 (TRIM68) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse E3 ubiquitin-protein ligase TRIM68 (TRIM68) Standard Curve.)
Related Product Information for TRIM68 elisa kit
Description: This Mouse E3 ubiquitin-protein ligase TRIM68 (TRIM68) ELISA Kit employs a two-site sandwich ELISA to quantitate TRIM68 in samples. An antibody specific for TRIM68 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRIM68 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRIM68 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRIM68 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TRIM68 contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene is expressed in many cancer cell lines. Its expression in normal tissues, however, was found to be restricted to prostate. This gene was also found to be differentially expressed in androgen-dependent versus androgen-independent prostate cancer cells.The deduced 485-amino acid protein has a calculated molecular mass of 56 kD. It has a RING finger domain, followed by a B box and a coiled-coil region. The deduced protein contains an N-terminal cys-his cluster, followed by a bipartite nuclear localization signal, a coiled-coil leucine zipper-like domain, and a C-terminal RET finger protein (TRIM27)-like domain.
Background: TRIM68 contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene is expressed in many cancer cell lines. Its expression in normal tissues, however, was found to be restricted to prostate. This gene was also found to be differentially expressed in androgen-dependent versus androgen-independent prostate cancer cells.The deduced 485-amino acid protein has a calculated molecular mass of 56 kD. It has a RING finger domain, followed by a B box and a coiled-coil region. The deduced protein contains an N-terminal cys-his cluster, followed by a bipartite nuclear localization signal, a coiled-coil leucine zipper-like domain, and a C-terminal RET finger protein (TRIM27)-like domain.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
E3 ubiquitin-protein ligase TRIM68
UniProt Protein Name
E3 ubiquitin-protein ligase TRIM68
Protein Family
UniProt Gene Name
Trim68
UniProt Synonym Gene Names
Rnf137
UniProt Entry Name
TRI68_MOUSE
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Product Notes
The Mouse TRIM68 trim68 (Catalog #AAA9718143) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718143 ELISA Kit recognizes Mouse TRIM68. It is sometimes possible for the material contained within the vial of "E3 Ubiquitin-Protein Ligase TRIM68 (TRIM68), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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