Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse E-Selectin (SELE) Standard Curve.)
Mouse E-Selectin (SELE) ELISA Kit | SELE elisa kit
Mouse E-Selectin (SELE) ELISA Kit
Gene Names
Sele; Elam; CD62E; ELAM-1; LECAM2; E-selectin
Reactivity
Mouse
Synonyms
E-Selectin (SELE); Mouse E-Selectin (SELE) ELISA Kit; SELE; RP1-117P20.2; CD62E; ELAM; ELAM1; ESEL; LECAM2; endothelial adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2; SELE elisa kit
Reactivity
Mouse
Specificity
Mouse E-Selectin (SELE) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SELE. No significant cross-reactivity or interference between Mouse SELE and analogues was observed.
Sequence Length
612
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse E-Selectin (SELE) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse E-Selectin (SELE) Standard Curve.)
Related Product Information for SELE elisa kit
Description: This Mouse E-Selectin (SELE) ELISA Kit employs a two-site sandwich ELISA to quantitate SELE in samples. An antibody specific for SELE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SELE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SELE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SELE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: CD62E is also known as endothelial leukocyte adhesion molecule 1 (ELAM1) or E selectin. It is a type I membrane protein that belongs to the selectin family of surface molecules (along with CD62L and CD62P). Selectins are C type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. CD62E (E selectin) is an endothelial cell specific selectin that is expressed on cytokine induced endothelial cells only after activation with proinflammatory cytokines. In vitro experiments have shown that IL1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of CD62E in an NFkB dependent signalling cascade. CD62E has been associated with blood vessel endothelium in diverse inflammatory situations.
Background: CD62E is also known as endothelial leukocyte adhesion molecule 1 (ELAM1) or E selectin. It is a type I membrane protein that belongs to the selectin family of surface molecules (along with CD62L and CD62P). Selectins are C type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. CD62E (E selectin) is an endothelial cell specific selectin that is expressed on cytokine induced endothelial cells only after activation with proinflammatory cytokines. In vitro experiments have shown that IL1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of CD62E in an NFkB dependent signalling cascade. CD62E has been associated with blood vessel endothelium in diverse inflammatory situations.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
E-selectin
NCBI Official Synonym Full Names
selectin, endothelial cell
NCBI Official Symbol
Sele
NCBI Official Synonym Symbols
Elam; CD62E; ELAM-1; LECAM2; E-selectin
NCBI Protein Information
E-selectin
UniProt Protein Name
E-selectin
Protein Family
UniProt Gene Name
Sele
UniProt Synonym Gene Names
Elam-1; ELAM-1; LECAM2
UniProt Entry Name
LYAM2_MOUSE
Uniprot Description
SELE: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Belongs to the selectin/LECAM family.
Protein type: Membrane protein, integral
Cellular Component: cortical cytoskeleton; extracellular space; membrane; perinuclear region of cytoplasm; integral to membrane; coated pit; caveola; lipid raft
Molecular Function: phospholipase binding; transmembrane receptor activity; carbohydrate binding; sialic acid binding
Biological Process: heterophilic cell adhesion; leukocyte adhesion; phospholipase C activation; positive regulation of leukocyte migration; positive regulation of receptor internalization; actin filament-based process; leukocyte tethering or rolling; cell adhesion; inflammatory response; signal transduction
Research Articles on SELE
Similar Products
Product Notes
The Mouse SELE sele (Catalog #AAA9717073) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717073 ELISA Kit recognizes Mouse SELE. It is sometimes possible for the material contained within the vial of "E-Selectin (SELE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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